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Anti-Alpha-II Spectrin Antibody (Previously Covance catalog# SIG-39702)

Pricing & Availability
Clone
D8B7 (See other available formats)
Regulatory Status
RUO
Other Names
Spectrin alpha chain, brain, alpha-II spectrin, fodrin alpha chain, spectrin, non-erythroid alpha chain, EL2, SPTA1
Previously
Covance Catalog# SIG-39702
Signet Catalog# 9702-02
Signet Catalog# 9702-05
Signet Catalog# 9702-10
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
D8B7_Ascites_AlphaIISpectrin_Antibody_1_081318
Western blot of anti-Alpha-II Spectrin antibody (clone D8B7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 20 µg of 3T3 cell lysate; Lane 6: 20 µg of HeLa cell lysate. The blot was incubated with the primary antibody at 1:2000 dilution overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • D8B7_Ascites_AlphaIISpectrin_Antibody_1_081318
    Western blot of anti-Alpha-II Spectrin antibody (clone D8B7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 20 µg of 3T3 cell lysate; Lane 6: 20 µg of HeLa cell lysate. The blot was incubated with the primary antibody at 1:2000 dilution overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • D8B7_Ascites_AlphaIISpectrin_Antibody_2_081318
    IHC staining of anti-Alpha-II Spectrin antibody (clone D8B7) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with the primary antibody at 1:1000 dilution overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • D8B7_Ascites_AlphaIISpectrin_Antibody_3_081318
    IHC staining of anti-Alpha-II Spectrin antibody (clone D8B7) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with the primary antibody at 1:1000 dilution overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • D8B7_Ascites_AlphaIISpectrin_Antibody_4_081318
    ICC staining of anti-Alpha-II Spectrin antibody (clone D8B7) on HeLa cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with the primary antibody at 1:1000 dilution overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 594 goat anti-Mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLongTM Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm
  • D8B7_Ascites_AlphaIISpectrin_Antibody_5_081318
    ICC staining of anti-Alpha-II Spectrin antibody (clone D8B7) on 3T3 cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with the primary antibody at 1:1000 dilution overnight at 4°C, followed by incubation with 2.5 µg/ml of Alexa Fluor® 594 goat anti-Mouse IgG for one hour at room temperature. Nuclei were counterstained with DAPI, and the slides were mounted with ProLongTM Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm
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803201 200 µL 581 CHF
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Description

The spectrins are a family of widely distributed cytoskeletal proteins which are involved in actin crosslinking. Spectrin α-chain heterodimerizes with tissue-specific β-chains. It is specifically expressed in nonerythrocytic cells. The protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in the gene are the cause of early infantile epileptic encephalopathy. Fodrin, a spectrin that seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus a candidate for the calcium-dependent movement of the cytoskeleton at the membrane.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Preparation
Ascites
Storage & Handling
Store at -20°C or below. Upon initial thawing, apportion into working aliquots and store at -20°C or below. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Do not store in frost-free freezers.
Application

WB - Quality tested
IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:2000 - 1:4000 dilution. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a dilution of 1:1000 is suggested. For immunocytochemistry, a dilution of 1:1000 is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone D8B7 is a specific marker for human, rat and mouse alpha II spectrin or fodrin. In cerebellar cell cultures, it labels axons and periphery of cell bodies consistent with localization of fodrin (reviewed in Bennett, 1993; Goodman, 1995 & De Matteis, 2000)4, 6, 8. It does not cross-react with alpha I (erythroid) spectrin SH3 domain.

This antibody was developed against a recombinant alpha II spectrin (non-erythroid spectrin or fodrin) SH3 domain. It was subsequently purified via ammonium sulfate precipitation.

Reactivity to Drosophila was only verified with the purified format.

Application References

(PubMed link indicates BioLegend citation)
  1. Konieczny P, et al. 2008. J Cell Biol. 181:667-81
  2. Xu J, et al. 2001. Brain Res. 898:171-7 (WB, IF)
  3. Xu J, et al. 2000. J Cell Science. 113:3805-14
  4. De Matteis MA and Morrow JS. 2000. J. Cell Science. 113:2331-43
  5. Swanson JA and Watts C. 1995. Trends Cell Biol. 5:424-427
  6. Goodman SR, et al. 1995. Brain Res Bull. 36:593-606
  7. Lamaze C and Schimid SL. 1995. Curr Opin Cell Biol. 7:573-580
  8. Bennett V and Gilligan DM. 1993. Annu Rev Cell Biol. 9:27-66
  9. Huang C, et al. 2017. J Neurosci. 37:11323-11334 PubMed
Product Citations
  1. Wang G et al. 2019. eLife. 8 pii: e38730. PubMed
  2. Miazek A, et al. 2021. Scientific Reports. 11(1):7312. PubMed
  3. Torii T, et al. 2020. The Journal of Cell Biology. 219(2):e201907048. PubMed
  4. Huang CY et al. 2017. The Journal of Neuroscience. 37(47):11323-11334 . PubMed
  5. Zhou R, et al. 2022. Nat Commun. 13:3196. PubMed
  6. Lubbers ER, et al. 2019. J Biol Chem. 294:9576. PubMed
  7. Hamdan H, et al. 2020. Nat Commun. 0.527777778. PubMed
  8. Huang CY et al. 2017. The Journal of Neuroscience. 37(47):11311-11322 . PubMed
  9. Liu Y, et al. 2019. Sci Adv. 5:eaav7803. PubMed
  10. Cousin MA, et al. 2021. Nat Genet. 53:1006. PubMed
RRID
AB_2564660 (BioLegend Cat. No. 803201)

Antigen Details

Structure
Alpha-II Spectrin is a 2472 amino acid protein with an expected molecular mass of 285 kD. A cleavage product of ~ 150 kD is observed in tissue lysates (1) (2).
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Presynaptic proteins, Scaffold Proteins
Antigen References
  1. Pike BR, et al. 2001. J Neurochem. 78(6):1297-306.
  2. Weiss ES, et al. 2009. Ann Thorac Surg. 88(2):543
Gene ID
6709 View all products for this Gene ID
UniProt
View information about alpha-II Spectrin on UniProt.org
Go To Top Version: 3    Revision Date: 03.12.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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