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Anti-BG-5 Antibody (Previously Covance catalog# SIG-3314)

Pricing & Availability
Clone
T174 (See other available formats)
Regulatory Status
RUO
Other Names
BG5
Previously
Signet Catalog# 314-01
Covance Catalog# SIG-3314
Isotype
Mouse IgG1, κ
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Product Citations
publications
T174_Purified_BG-5_Antibody_IHC_032615
IHC staining of anti-BG-5 antibody (clone T174) on formalin-fixed paraffin-embedded human antrum tissue. The tissue was incubated with a 1:40 dilution of the primary antibody for 20 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • T174_Purified_BG-5_Antibody_IHC_032615
    IHC staining of anti-BG-5 antibody (clone T174) on formalin-fixed paraffin-embedded human antrum tissue. The tissue was incubated with a 1:40 dilution of the primary antibody for 20 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
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922202 1 mL 402 CHF
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Description

BG-5, also known as blood group Lewis A, is found in gastrointestinal tumors, colorectal tumors, and pancreatic cancer. It is expressed in colonic epithelial cells. Blood group related antigens include A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain group of antigens. These are a group of mucin-type carbohydrate determinants found on erythrocytes, a subset of epithelial cells, and in secretions.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was developed against the SK-CO-10 colon cancer cell line. It is specific for the Lewis (a) (type 1 chain).
Formulation
Phosphate-buffered solution with BSA + 0.09% NaN3.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
The antibody was purified by ammonium sulfate precipitation.
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-P - Quality tested
ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining.  For immunohistochemistry, a dilution of 1:40 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Monteiro MA, et al. 1998. J Biol Chem. 273: 11533-43. (ELISA)
Product Citations
  1. Moya-Gonzalvez EM, et al. 2022. Microbiol Spectr. 10:e0177522. PubMed
RRID
AB_2565410 (BioLegend Cat. No. 922202)

Related FAQs

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Go To Top Version: 6    Revision Date: 02.08.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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