Anti-CD49C Integrin alpha3 Antibody anti-CD49C (Integrin alpha3)

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Clone: P1B5 (See other available formats)
Regulatory Status: RUO
Other Names: GAP-B3, GAPB3, ILNEB, MSK18, VCA-2, VL3A, VLA3a, FRP-2, galactoprotein B3, VLA-3 subunit alpha, CD49 antigen-like family member C, antigen identified by monoclonal antibody J143, very late activation protein 3 receptor, alpha-3 subunit
Previously: Covance Catalog# MMS-490R
Isotype: Mouse IgG1, κ
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Product Citations: publications

IHC staining of anti-CD49C (Integrin α3) antibody (clone P1B5) on frozen human kidney tissue. The tissue was incubated with a 1:250 dilution of the primary antibody for 60 minutes at room temperature. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm

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921601

100 µL

256 CHF

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Description

CD49c is a 150 kD α integrin chain known as α3 integrin or VLA-3 α chain. It is a type I transmembrane glycoprotein which is proteolytically cleaved into two disulfide linked fragments of 125 kD and 30 kD. CD49c forms a heterodimer with integrin β1 (α3β1, CD49c/CD29, VLA-3) and is expressed by many types of adhesion cells, such as endothelial cells, epithelial cells, and dermal fibroblasts. Weak expression has been reported on leukocytes. VLA-3 plays a role in cell-cell and cell-matrix adhesion through binding Kalinin, collagen, laminin-1, laminin-5, entactin, and fibronectin.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: The P1B5 monoclonal antibody was generated against human HT1080 cells.
Preparation: Ascites
Concentration: The concentration is not quantified as this product is sold as undiluted crude mouse ascites fluid. The concentration might vary from lot-to-lot and an estimated concentration would be 1-3 mg/ml.
Storage & Handling: Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application: IHC-F - Quality tested
FC - Verified
IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a dilution range of 1:250 - 1:500 is suggested. For flow cytometric staining, the suggested dilution of this reagent is between 1:250 - 1:500. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody recognizes the a3 subunit.
RRID: AB_2565403 (BioLegend Cat. No. 921601)

Antigen Details

Structure: CD49c is a 150 kD type I transmembrane glycoprotein
Distribution: Cellular distribution: Plasma membrane.
Tissue distribution: Endothelial cells, epithelial cells, and dermal fibroblast.
Biology Area: Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family: Adhesion Molecules
Gene ID: 3675 View all products for this Gene ID
UniProt: View information about CD49c on UniProt.org

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Related Protocols

Immunohistochemistry Protocol for Frozen Sections

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Other Formats

View All CD49C (Integrin α3) Reagents Request Custom Conjugation

Description	Clone	Applications
Anti-CD49C (Integrin α3)	P1B5	IHC-F,FC,IP
Purified anti-CD49C (Integrin α3)	P1B5	IHC-F,ICC,FC,IP
Alexa Fluor® 647 anti-CD49C (Integrin α3)	P1B5	IHC-F

Certificate of Analysis

Go To Top Version: 3 Revision Date: 03.21.2019

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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