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Anti-human CD3 FITC/CD4 PE/CD45 PerCP Cocktail

Pricing & Availability
Clone
SK7; SK3; HI30;
Regulatory Status
RUO
Isotype
Mouse IgG1, κ (all clones)
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SK7-SK3-HI30_Cocktail_1_FC_081915
Human peripheral blood lymphocytes were stained with anti-human CD3 FITC/CD4 PE/CD45 PerCP cocktail. The top image shows SSC versus CD45+ profile ungated. The bottom image (CD3 versus CD4) image is gated on CD45+ lymphocytes as shown in the top image.
  • SK7-SK3-HI30_Cocktail_1_FC_081915
    Human peripheral blood lymphocytes were stained with anti-human CD3 FITC/CD4 PE/CD45 PerCP cocktail. The top image shows SSC versus CD45+ profile ungated. The bottom image (CD3 versus CD4) image is gated on CD45+ lymphocytes as shown in the top image.
  • SK7-SK3-HI30_Cocktail_2_FC_08192015
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368302 50 tests 323 CHF
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Description

CD3: SK7 antibody reacts with a combinatorial epitope of CD3ε, a 20 kD chain of the CD3/T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells, and some thymocytes.
CD4: SK3 antibody reacts with CD4, a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages.
CD45: HI30 antibody reacts with CD45, a 180-240 kD single chain type I membrane glycoprotein which is also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
This reagent is a combination of FITC conjugated SK7, PE conjugated SK3, and PerCP conjugated HI30 at optimal concentration for three-color flow cytometric analysis.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood.

Application Notes

This cocktail has been optimized to be used in a lyse-wash and lyse-no wash assay.

Note:
Single color controls are required to perform instrument compensation and are not included in the kit.

RRID
AB_2566176 (BioLegend Cat. No. 368302)

Antigen Details

Distribution

T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells, some thymocytes, and hematopoietic cells (not expressed in circulating erythrocytes or platelets).

Cell Type
Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
Antigen References

1. Kan EA, et al. 1983. J. Immunol. 131:536.
2. Wood GS, et al. 1985. Am. J. Pathol. 120:371.
3. van Dongen JJ, et al. 1988. Blood 71:603.
4. Center D, et al. 1996. Immunol. Today 17:476.
5. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.
6. Knapp W, et al. (1989) Leucocyte Typing IV. New York, NY: Oxford University Press.
7. Kishihara K, et al. 1993. Cell 74:143.
8. Esser M, et al. 2001. J. Virol. 75:6173.

Gene ID
916 View all products for this Gene ID 920 View all products for this Gene ID 5788 View all products for this Gene ID

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 08.19.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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