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Anti-TYRP1 (MEL-5) Antibody (Previously Covance catalog# SIG-38150)

Pricing & Availability
Clone
Ta99 (See other available formats)
Regulatory Status
RUO
Other Names
CAS2, CATB, GP75, OCA3, TRP, 5,6-dihydroxyindole-2-carboxylic acid oxidase, TRP1, TRP-1, catalase B, DHICA oxidase, glycoprotein 75, melanoma antigen gp75
Previously
Signet Catalog# 8150-01
Covance Catalog# SIG-38150
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
Ta99_Purified_TYRP1_Antibody_IHC_032615
Staining of MEL-5 (Ta99), on frozen human melanoma tissue.
  • Ta99_Purified_TYRP1_Antibody_IHC_032615
    Staining of MEL-5 (Ta99), on frozen human melanoma tissue.
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917801 1 mL 392 CHF
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Description

Tyrosinase-related protein 1, also known as TYRP1 or MEL-5, is an enzyme found in melanocytes and is involved in the synthesis of melanin. TYRP1 stabilizes tyrosinase and modulates its catalytic activity. It is also involved in the maintenance of melanosome structures, as well as the proliferation and cell death of melanocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Ta99 was developed using a SK-MEL-23 Melanoma cell line.
Formulation
Phosphate-buffered solution with BSA + 0.09% NaN3.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-F - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
• IHC: ≥1:20 (concentrated format) with Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).

Tissue Sections: The preferred specimens for this antibody are frozen tissue sections and/or cytocentrifuge preparations fixed 10 minutes in 4°C acetone.
Pretreatment: Not required.
Incubation: 60 minutes at room temperature

Application Notes

This antibody is effective in immunohistochemistry (IHC).

Positive tissue (human): Melanoma, normal skin

The BioLegend Mel 5 (Ta99) monoclonal antibody recognizes gp75, an abundant intracellular glycoprotein that is expressed in the melanosomes of human melanocytes and melanomas.

Application References

(PubMed link indicates BioLegend citation)
  1. Gross EA, et al. 1999. Archives Dermatol. 135:15.
  2. Bhawan, J, Cao SL. 1999. Am. J. Dermatopathol. 21:225.
  3. Kemp EH, et al. 1998. Br J Dermatol. 139:798.
  4. Burkhalter A, White WL. 1997. Am J Dermapathol. 19:303.
  5. Andersen WK, et al. 1997. J Am Acad Dermatol. 36:444.
  6. Bhawan J. 1997. Melanoma Res. 7:43.
  7. Zhao H, et al. 1996. J Invest Dermatol. 106:744.
  8. Cui J, et al. 1995. Pigment Cell Res. 8:53.
  9. Vijayasaradhi S, Houghton AN. 1991. Int J Cancer. 47:298.
  10. Thomson TM, et al. 1988. J Invest Dermatol. 90:459.
Product Citations
  1. Chow SYA, et al. 2022. Cell Rep. 40:111366. PubMed
  2. Ruhland MK, et al. 2020. Cancer Cell. 37(6):786-799.e5. PubMed
  3. Zurina IM, et al. 2020. Front Bioeng Biotechnol. 0.708333333. PubMed
  4. Jackson E, et al. 2019. Int J Mol Sci. 5.099305556. PubMed
RRID
AB_2565201 (BioLegend Cat. No. 917801)

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Go To Top Version: 7    Revision Date: 02.08.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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