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Anti-yeast Sir3 Antibody

Pricing & Availability
Clone
185D (See other available formats)
Regulatory Status
RUO
Other Names
Silent information regulator 3, CMT1, MAR2, STE8
Previously
NeoClone Catalog# W0036
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
185D_Ascites_yeastSir3_Antibody_WB_051116
Western blot analysis of 50 ng of recombinant yeast Sir3 (lane 1), 15 µg E. coli BL21 (lane 2), yeast (saccharomyces cerevisiae) (lane 3) and HeLa (lane 4) using anti-yeast Sir3 (clone 185D) antibody followed by HRP goat anti-mouse IgG antibody.
  • 185D_Ascites_yeastSir3_Antibody_WB_051116
    Western blot analysis of 50 ng of recombinant yeast Sir3 (lane 1), 15 µg E. coli BL21 (lane 2), yeast (saccharomyces cerevisiae) (lane 3) and HeLa (lane 4) using anti-yeast Sir3 (clone 185D) antibody followed by HRP goat anti-mouse IgG antibody.
Cat # Size Price Quantity Check Availability Save
669103 100 µL 303 CHF
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Description

SIR1, SIR2, SIR3, and SIR4 are proteins that are required for transcriptional repression of the silent mating type loci, HML and HMR. SIR2, SIR3, and SIR4 proteins repress multiple loci by modulating chromatin structure, which involves compaction of chromatin fiber into a more condensed form.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Yeast
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Preparation
Dried ascites
Storage & Handling
Store lyophilized antibody at -20°C.
Application

WB - Quality tested
ELISA - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. To reconstitute, add 100 µl of ddH2O to the tube containing antibody and mix to dissolve. For Western blotting, the suggested dilution is 1:1000 after reconstitution. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Previously item# W0036 from Neoclone.

RRID
AB_2564540 (BioLegend Cat. No. 669103)

Antigen Details

Structure
Sir3 is a 111 kD Saccharomyces cervisiae transcription repressor and is a member of the Sir (Silent Information Regulator) family.
Biology Area
Cell Biology, Transcription Factors
Gene ID
851163 View all products for this Gene ID
UniProt
View information about Sir3 on UniProt.org
Go To Top Version: 4    Revision Date: 09.19.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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