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APC anti-human CD24 Antibody

Pricing & Availability
Clone
W20001B (See other available formats)
Regulatory Status
RUO
Other Names
Heat stable antigen (HSA), nectadrin, Ly-52
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
W20001B_APC_CD24_Antibody_111722
Human peripheral blood lymphocytes were stained with anti-human CD24 (clone W20001B) APC (left) or mouse IgG2b, κ APC isotype control (right). Cells were costained with anti-human CD19 FITC.
  • W20001B_APC_CD24_Antibody_111722
    Human peripheral blood lymphocytes were stained with anti-human CD24 (clone W20001B) APC (left) or mouse IgG2b, κ APC isotype control (right). Cells were costained with anti-human CD19 FITC.
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382603 25 tests 147 CHF
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382604 100 tests 347 CHF
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Description

CD24 is a 35-45 kD glycosylphosphatidylinositol (GPI)-linked protein also known as heat stable antigen (HSA), BA-1, Ly-52, and nectadrin. It is expressed on the surface of B cells (but not plasma cells), granulocytes, follicular dendritic cells, and epithelial cells. CD24 may play a role in the regulation of B-cell proliferation and maturation. CD24 crosslinking induces a Ca2+ flux in mature B cells. CD24 has been shown to interact with CD62P (P-selectin).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CD24 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone W20001B can partially block binding of clone ML5 on target cells and is partially blocked by ML5.

Product Citations
  1. Wei Y, et al. 2023. iScience. 108022:26. PubMed
RRID
AB_2927986 (BioLegend Cat. No. 382603)
AB_2927986 (BioLegend Cat. No. 382604)

Antigen Details

Structure
35-45 kD glycosylphosphatidylinos GPI-linked protein
Distribution

B lymphocytes, granulocytes, differentiating neurons, and epithelial cells

Function
Regulates B-cell proliferation and activation
Ligand/Receptor
P-Selectin and Siglec-10
Cell Type
B cells, Epithelial cells, Granulocytes, Lymphocytes, Neural Stem Cells, T cells, Thymocytes
Biology Area
Cancer Biomarkers, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immuno-Oncology, Immunology, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Schlossman S, et al. 1995. Leukocyte Typing V. Oxford University Press. New York.
  2. Fang X, et al. 2010. Cellular and Molecular Immunology. 7:100-3.
Gene ID
100133941 View all products for this Gene ID
UniProt
View information about CD24 on UniProt.org
Go To Top Version: 1    Revision Date: 11.17.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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