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APC anti-human CD254 (TRANCE, RANKL) Antibody

Pricing & Availability
Clone
MIH24 (See other available formats)
Regulatory Status
RUO
Other Names
TRANCE (TNF-related activation-induced cytokine receptor), CD254, RANK-L (receptor activator of NF-κB ligand), OPGL (Osteoprotegerin ligand), SOFA, TNFSF-11
Isotype
Mouse IgG2b, κ
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Product Citations
publications
MIH24_APC_CD254_Antibody_FC_092514
Human RANKL transfected P815 cell line was stained with CD254 (clone MIH24) APC (filled histogram) or mouse IgG2b, κ APC isotype control (open histogram).
  • MIH24_APC_CD254_Antibody_FC_092514
    Human RANKL transfected P815 cell line was stained with CD254 (clone MIH24) APC (filled histogram) or mouse IgG2b, κ APC isotype control (open histogram).
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347507 25 tests 166 CHF
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347508 100 tests 362 CHF
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Description

Human CD254, also known as RANKL, TRANCE, and OPGL, is a type II transmembrane protein in the TNF superfamily that has two forms: a membrane-anchored protein and a secreted protein. Its extracellular domain is related to TRAIL, FasL, and TNF. CD254 is expressed on osteoblasts and activated T cells. It has been implicated in the regulation of the interactions between T cells and dendritic cells. It plays an important role in osteoblast differentiation and bone resorption. CD254 activates JNK and NF-κB and induces the expression of IL-1, IL-6, IL-12, and IL-15 in dendritic cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
RANKL-transfected L cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats of this clone) include: ELISA and immunohistochemical staining of paraffin-embedded tissue sections2.

Application References

(PubMed link indicates BioLegend citation)
  1. Kanamaru F, et al. 2004. Immunol. Lett. 94:239.
  2. Mueller A, et al. 2014. Arthritis Res. Ther. 16:R55. (IHC) PubMed
Product Citations
  1. Dewulf J, et al. 2021. Cancers (Basel). 13: . PubMed
  2. Marini C,et al. 2017. Sci Rep. . 10.1038/s41598-017-12761-1. PubMed
  3. Loftus A, et al. 2020. J Bone Miner Res. 35:396. PubMed
RRID
AB_2564322 (BioLegend Cat. No. 347507)
AB_2564322 (BioLegend Cat. No. 347508)

Antigen Details

Structure
A TNF superfamily member.
Distribution

Expressed on osteoblasts and activated T cells.

Function
Plays an important role in osteoblast differentiation and bone resorption, regulates the interactions between T cells and dendritic cells, and T cells and B cells.
Ligand/Receptor
RANK, OPG.
Cell Type
T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokines/Chemokines
Antigen References

1. Wiethe C, et al. 2003. J. Immunol. 171:4121
2. Page G, et al. 2005. Arthritis Rheum. 52:2307
3. Ji JD, et al. 2009. J. Immunol. 183:7223

Gene ID
8600 View all products for this Gene ID
UniProt
View information about CD254 on UniProt.org
Go To Top Version: 1    Revision Date: 09.30.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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