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APC anti-human HB-EGF Antibody

Pricing & Availability
Clone
3H4 (See other available formats)
Regulatory Status
RUO
Other Names
Heparin-binding EGF-like growth factor, HB-EGF-like growth factor
Isotype
Mouse IgG1, κ
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Product Citations
publications
3H4_APC_HB-EGF_Antibody_1_042424
NUGC-3 cells (human gastric cancer cells) were stained with anti-human HB-EGF (clone 3H4) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
  • 3H4_APC_HB-EGF_Antibody_1_042424
    NUGC-3 cells (human gastric cancer cells) were stained with anti-human HB-EGF (clone 3H4) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
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383705 25 tests 196 CHF
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383706 100 tests 409 CHF
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Description

Heparin-binding EGF-like growth factor (HB-EGF) is a member of the EGF family of growth factors. Human HB-EGF was initially identified as a protein of 22 kD secreted by macrophage-like U937 cells. It belongs to the EGF family of proteins that includes EGF, TGF-α, HB-EGF, epigen, epiregulin, betacellulin, neuroregulin, and tomoregulin. All the EGF family members are synthesized as type I membrane protein precursors, which can undergo proteolytic cleavage at the plasma membrane to release a mature soluble ectodomain. It has been suggested that various metalloproteinases participate in the shedding of HB-EGF, such as MMP-3, MMP-7, ADAM9, ADAM10, ADAM12, and ADAM17. The ectodomain shedding is stimulated by phorbol esters, calcium ionophore, lysophosphatidic acid, and IL-1β. In addition, nardilysin, a metalloendopeptidase of the M16 family, binds HB-EGF and enhances its shedding through activation of TACE (ADAM17). The membrane-anchored HB-EGF acts in cell-cell and cell-extracellular matrix interactions. In addition, the membrane HB-EGF is the receptor for diphtheria toxin that can be internalized and induce apoptotic death. HB-EGF plays a crucial role in cardiac valvulogenesis. Newborn knockout mice have malformed semilunar and atrioventricular heart valves and poorly differentiated lungs. HB-EGF is overexpressed in several types of cancer such as pancreatic carcinoma, ovarian cancer, and gastric carcinoma. HB-EGF is also involved in cancer metastasis and invasion of ovarian cancer, head and neck cancer, and thyroid carcinoma cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human HB-EGF, extracellular domain (recombinant)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Hamaoka M, et al. 2010. J Biochem. 148:55.
RRID
AB_3106177 (BioLegend Cat. No. 383705)
AB_3106177 (BioLegend Cat. No. 383706)

Antigen Details

Distribution

Monocytes, macrophages, T cells, neutrophils, eosinophils, myeloid leukemia blasts, myeloma cells, vascular smooth muscle cells, endothelial cells, and normal or neoplastic epithelial cells

Function
HB-EGF is associated with proliferation and differentiation of stromal cells, apoptosis, and morphogenesis. It participates in inflammation, wound healing, atherosclerotic plaque progression, and cardiac valvulogenesis. TNF-α and IL-1β induces HB-EGF in endothelial cells.
Interaction
Fibroblast, smooth muscle cells, monocytes/macrophages, endothelial cells, astrocytes, keratinocytes, normal and neoplastic epithelial cells
Ligand/Receptor
EGFR (ErbB1) and ErbB4; also binds to heparan-sulphate proteoglycans
Cell Type
Epithelial cells, Macrophages, T cells
Biology Area
Angiogenesis, Cancer Biomarkers
Molecular Family
Growth Factors
Antigen References
  1. Higashiyama S, et al. 1991. Science. 251:936.
  2. Hirata M, et al. 2001. Biochem. Biophys. Res. Commun. 283:915.
  3. Jackson LF, et al. 2003. EMBO J. 22:2704.
  4. Nishi E, et al. 2006. J. Biol. Chem. 281:31164.
  5. Yotsumoto F, et al. 2008. Biochem. Biophys. Res. Commun. 365:555.
  6. Ota I, et al. 2013. Oncol Rep. 30:1593.
Gene ID
1839 View all products for this Gene ID
UniProt
View information about HB-EGF on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04.24.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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