APC anti-human MCP-1 Antibody anti-MCP-1

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Clone: 5D3-F7 (See other available formats)
Regulatory Status: RUO
Other Names: Monocyte chemoattractant protein-1, Monocyte chemoattractant and activating factor (MCAF), JE, Small inducible cytokine A2 (SCYA2), HC-11, P6, Smooth muscle cell chemotactic factor (SMC-CF), CCL2
Isotype: Mouse IgG1, κ
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5D3-F7_APC_MCP-1_Antibody_050118 — IFN-γ primed human peripheral blood monocytes were stimulated with LPS overnight (in the presence of monensin), then surface stained with CD14 PE and intracellularly stained with MCP-1 APC (left) or mouse IgG1, κ APC isotype control (right).

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502611

25 tests

137 CHF

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502612

100 tests

267 CHF

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Description

Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 5D3-F7 antibody reacts with human monocyte chemoattractant protein-1 (MCP-1).

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human MCP-1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: ELISA or ELISPOT Detection¹: The biotinylated 5D3-F7 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified 2H5 antibody (Cat. No. 505902/505906) as the capture antibody.
ELISA or ELISPOT Capture: The purified 5D3-F7 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 2H5 antibody (Cat. No. 505908) as the detection antibody. The LEAF™ purified antibody (Cat. No. 502607) is suggested for ELISPOT capture.
Additional reported applications (for the relevant formats) include: intracellular flow cytometry², immunoprecipitation^1,3, Western blotting¹, and immunohistochemical staining¹.
RRID: AB_2734488 (BioLegend Cat. No. 502611)
AB_2734488 (BioLegend Cat. No. 502612)

Antigen Details

Structure: CC chemokine; dimer; 13.8 kD (Mammalian)
Bioactivity: Chemoattractant; regulate adhesion molecule expression, cytokine production in monocytes; proliferation/activation of CC-chemokine activated killer cells
Cell Sources: Monocytes, vascular endothelial cells, smooth muscle, glomerular mesangial cells
Cell Targets: Monocytes, basophils, T cells, NK cells, immature dendritic cells
Receptors: CCR2; CCR5; CCR10; D6; possibly L-CCR
Cell Type: Embryonic Stem Cells
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Bischoff S, et al. 1992. J. Exp. Med. 175:1271.
3. Charo I, et al. 1994. P. Natl. Acad. Sci. USA 91:2752.
4. Jiang Y, et al. 1992. J. Immunol. 148:2423.
Regulation: Upregulated by IL-1, TNF-α, PDGF, TGF-β
Gene ID: 6347 View all products for this Gene ID
UniProt: View information about CCL2 on UniProt.org

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Other Formats

View All CCL2 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-human MCP-1	5D3-F7	ICFC
Purified anti-human MCP-1	5D3-F7	ELISA Capture,WB,ELISA,ELISPOT Detection,ICFC,IHC,IP
Biotin anti-human MCP-1	5D3-F7	ELISA Detection,ELISPOT Detection,ICFC
APC anti-human MCP-1	5D3-F7	ICFC
PE/Cyanine7 anti-human MCP-1	5D3-F7	ICFC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 05.01.2018

For Research Use Only. Not for diagnostic or therapeutic use.

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