APC anti-human MRP-14 S100A9 Antibody anti-MRP-14

Pricing & Availability

Clone: MRP 1H9 (See other available formats)
Regulatory Status: RUO
Other Names: S100A9, Migration inhibitory factor-related proteins-14, Calgranulin-B, Leukocyte L1 complex heavy chain, Calprotectin L1H subunit
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human peripheral blood granulocytes were stained with CD15 Pacific Blue™, fixed, permeabilized, and then intracellularly stained with MRP-14 (clone MRP 1H9) APC (top) or mouse IgG1, κ APC isotype control (bottom).

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350707

25 tests

142 CHF

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350708

100 tests

314 CHF

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Description

MRP-14, also known as S100A9, belongs to the S100 family of proteins containing 2-EF-hand calcium-binding motifs. It forms non-covalently associated complexes with another S100 family member, MRP-8. MRP14 is expressed in neutrophils and monocytes; it also has been found in ileal tissue eosinophils. The S100A8/A9 complex has been shown to play a role during calcium-dependent activation as well as have antibacterial, antifungal, immunomodulating, and anti-proliferative effects. It also functions as a chemoattractant factor for neutrophils and has been considered an endogenous activator of Toll-like receptor 4. S100A9 has been identified as a marker for CD14(+) HLA-DR(-/low) MDSCs.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human MRP-14
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: This clone has been published as 1H9.

Additional reported applications (for the relevant formats) include: Western blotting¹ and immunohistochemical staining with paraffin-embedded tissue sections^1,2.
RRID: AB_2564010 (BioLegend Cat. No. 350707)
AB_2564010 (BioLegend Cat. No. 350708)

Antigen Details

Structure: Belongs to S100 family of proteins containing 2-EF-hand calcium -binding motifs, forms non-covalently associated complexes with another S100 family member MRP-8
Distribution: MDSCs, neutrophils, monocyte, and ileal tissue eosinophils
Function: Calcium-dependent activation, immunomodulating and antiproliferative, endogenous activator of Toll-like receptor 4
Interaction: Calcium
Cell Type: Neutrophils, Monocytes, Eosinophils
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family: Adhesion Molecules
Antigen References: 1. Loser K, et al. 2010. Nat. Med. 16:713.
2. Cheng P, et al. 2008. J. Exp. Med. 205:2235.
3. Leukert N, et al.2006. J. Mol. Biol. 359:961.
4. Lagasse E, et al. 1988. Mol. Cell. Biol. 8:2402.
5. Zhao F, et al. 2012. Immunology. 136:176.
Gene ID: 6280 View all products for this Gene ID
UniProt: View information about MRP-14 on UniProt.org

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Other Formats

View All MRP-14 Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-human MRP-14 (S100A9)	MRP 1H9	ICFC
PE anti-human MRP-14 (S100A9)	MRP 1H9	ICFC
APC anti-human MRP-14 (S100A9)	MRP 1H9	ICFC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 07.23.2014

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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