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- Clone
- QA20B12 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- α/β TCR, TCR α/β
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood lymphocytes were stained with anti-human (clone UCHT1) CD3 FITC and anti-human TCR α/β recombinant (clone QA20B12) APC (left) or mouse IgG1, κ APC isotype control (right).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 380803 | 25 tests | 105 CHF | ||||
| 380804 | 100 tests | 240 CHF | ||||
The α/β T-cell receptor (TCR) recognizes a peptide bound to MHC leading to T cell activation. α/β TCR expressing T cells comprise more than 90% of CD3+ T cells in peripheral blood. The clone QA20B12 antibody only reacts with the α/β TCR, not the γ/δ TCR.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Red Laser (633 nm)
- Application Notes
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Clone QA20B12 can completely block the binding of clone IP26 on target cells.
- RRID
-
AB_3662280 (BioLegend Cat. No. 380803)
AB_3662280 (BioLegend Cat. No. 380804)
Antigen Details
- Structure
- Ig superfamily
- Distribution
-
T cells, thymocytes
- Function
- Antigen recognition, T cell activation
- Interaction
- CD3
- Ligand/Receptor
- Peptide bound to MHC
- Cell Type
- T cells, Thymocytes, Tregs
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
- Marchalonis JJ, et al. 2002. J Mol Recognit. 15:260-71.
- Gene ID
- 6955 View all products for this Gene ID 6957 View all products for this Gene ID
- UniProt
- View information about TCR alpha/beta on UniProt.org
Other Formats
View All TCR α/β Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human TCR α/β Recombinant Antibody | QA20B12 | FC |
| APC anti-human TCR α/β Recombinant Antibody | QA20B12 | FC |
| PE anti-human TCR α/β Recombinant Antibody | QA20B12 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human TCR α/β Recombinant Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
APC anti-human TCR α/β Recombinant Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
PE anti-human TCR α/β Recombinant Antibody
Human peripheral blood lymphocytes were stained with anti-hu...
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