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APC anti-mouse B7-H4 (B7S1, B7X) Antibody

Pricing & Availability
Clone
HMH4-5G1 (See other available formats)
Regulatory Status
RUO
Other Names
B7H4, B7S1, B7-S1, B7x
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
HMH4-5G1_APC_B7-H4_Antibody_FC_121214
B7-H4 transfected 293 cells were stained with B7-H4 (clone HMH4-5G1) APC (filled histogram) or Armenian hamster IgG APC isotype control (open histogram).
  • HMH4-5G1_APC_B7-H4_Antibody_FC_121214
    B7-H4 transfected 293 cells were stained with B7-H4 (clone HMH4-5G1) APC (filled histogram) or Armenian hamster IgG APC isotype control (open histogram).
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139407 25 µg 142 CHF
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139408 100 µg 374 CHF
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Description

B7-H4 is a newly discovered B7 family member that negatively regulates T cell immunity. In vitro, B7-H4 inhibits CD4+ and CD8+ T cell proliferation, cytokine production, and generation of alloreactive cytotoxic T-cells (CTLs). In vivo, blockage of endogenous B7-H4 by specific monoclonal antibodies promotes T cell responses. A new reported function of B7-H4 is as an important negative regulator of innate immunity through growth inhibition of neutrophils. It has been reported that B7-H4 is not constitutively expressed on peripheral tissues but can be induced to express on T cells, B cells, macrophages, and dendritic cells. B7-H4 is expressed on some tumor cancer cells. The role of B7-H4 in tumor progression may be to transform precancerous cells and then protect them from immunosurveillance. Although B- and T-lymphocyte attenuator (BTLA) was proposed to be the receptor for B7-H4, further studies are needed to identify the inhibitory receptor of B7-H4.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse B7-H4 human IgG1 Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Product Citations
  1. Chen L, et al. 2020. Cancer Immunol Res. 0.457638889. PubMed
RRID
AB_2565217 (BioLegend Cat. No. 139407)
AB_2565217 (BioLegend Cat. No. 139408)

Antigen Details

Structure
Belongs to the B7 family, contains two extracellular Ig-like domains.
Distribution

Induced to express on T cells, B cells, macrophages, and dendritic cells. It is also expressed on some tumor cancer cells.

Function
Inhibit T cell activation, cell cycle progression, and cytokine production.
Cell Type
B cells, Dendritic cells, Macrophages, T cells
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Antigen References

1. Choi IH, et al. 2003. J. Immunol. 171:4650.
2. Prasad DV, et al. 2003. Immunity 18:863.
3. Kryczek I, et al. 2006. J. Exp. Med. 203:871.

Gene ID
242122 View all products for this Gene ID
UniProt
View information about B7-H4 on UniProt.org
Go To Top Version: 2    Revision Date: 07.08.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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