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APC anti-mouse CD21/CD35 (CR2/CR1) Antibody

Pricing & Availability
Clone
7E9 (See other available formats)
Regulatory Status
RUO
Other Names
CR2/CR1
Isotype
Rat IgG2a, κ
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Product Citations
publications
7E9_APC_011408
C57BL/6 mouse splenocytes stained with 7E9 APC
  • 7E9_APC_011408
    C57BL/6 mouse splenocytes stained with 7E9 APC
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123411 25 µg 119 CHF
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123412 100 µg 326 CHF
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Description

CD21, also known as CR2 (complement receptor 2) and C3d receptor, binds C3d and iC3b. It is also a receptor of Epstein-Barr virus. CD35, also known as CR1, binds C3b, iC3b, C4b, and iC4b. CD21/CD35 is primarily expressed on B lymphocytes, mast cells, follicular dendritic cells, macrophages, and activated granulocytes. CD21/CD35 forms part of the B-cell antigen receptor complex with CD19 and CD81 and is involved in signal transduction.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CD35/CFA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Boackle S, et al. 2001 Immunity 15:775.
  2. de Andres B, et al. 2012. J. Immunol. 189:2300. PubMed
  3. Chiu YK, et al. 2014. J Immunol. 193:2207. PubMed
  4. Koening PA, et al. 2014. J Biol Chem. 289:34490. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  2. Chei S, et al. 2020. Front Nutr. 7:583186. PubMed
  3. Laffleur B, et al. 2022. Sci Immunol. 7:eabn2738. PubMed
  4. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
  5. Lütge M, et al. 2023. Nat Immunol. . PubMed
  6. Wu J, et al. 2022. Nat Commun. 13:7321. PubMed
  7. Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed
  8. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  9. Petursdottir D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01699. PubMed
  10. Gómez-Díaz C, et al. 2021. iScience. 24:103241. PubMed
  11. Parthasarathy R, et al. 2021. Front Immunol. 12:758407. PubMed
  12. Sarapulov AV, et al. 2020. Front Immunol. 11:599. PubMed
  13. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  14. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  15. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  16. Rodda L, et al. 2015. J Immunol. 195: 4781 - 4791. PubMed
  17. Fennell LM, et al. 2020. EMBO J. 39:e103303. PubMed
  18. Zhu Y, et al. 2022. Clin Transl Med. 12:e887. PubMed
  19. Yunk L, et al. 2009. J Immunol. 183:452. PubMed
  20. Chiu Y, et al. 2014. J Immunol. 193:2207. PubMed
  21. Wang YH, et al. 2019. Front Immunol. 10:1909. PubMed
  22. Xiao S, et al. 2020. Cell Reports. 32(2):107892. PubMed
  23. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  24. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  25. Koenig P, et al. 2014. J Biol Chem. 289:34490. PubMed
  26. Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
  27. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  28. Andrés B, et al. 2012. J Immunol. 189:2300. PubMed
  29. Zane N, et al. 2012. J Immunol. 188:3009. PubMed
  30. Jing Y, et al. 2019. J Allergy Clin Immunol. 144:1377. PubMed
  31. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  32. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  33. Silva N, et al. 2016. J Immunol. 196: 2591 - 2601. PubMed
RRID
AB_940395 (BioLegend Cat. No. 123411)
AB_940395 (BioLegend Cat. No. 123412)

Antigen Details

Structure
145 kD/190 kD type I transmembrane glycoprotein
Distribution

B cells, follicular dendritic cells

Function
Signal transduction
Ligand/Receptor
Associated with CD19/CD81. CD21 binds C3d, iC3b, and EBV; CD35 binds C3b, iC3b, C4b, and iC4b.
Cell Type
B cells, Dendritic cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kozono Y, et al. 1998. J. Immunol. 160:1562.
2. Shimizu I, et al. 2007. Blood 109:1773.
3. Roozendaal R and MC. Carroll. 2007. Immunol. Rev. 219:157.

Gene ID
12902 View all products for this Gene ID 12946 View all products for this Gene ID
UniProt
View information about CD21/CD35 on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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