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APC anti-mouse CD272 (BTLA) Antibody

Pricing & Availability
Clone
8F4 (See other available formats)
Regulatory Status
RUO
Other Names
BTLA, B and T lymphocyte attenuator
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_8F4_APC_012110
C57BL/6 mouse splenocytes stained with 8F4 APC and CD45R/B220 (RA3-6B2) PE
  • A_8F4_APC_012110
    C57BL/6 mouse splenocytes stained with 8F4 APC and CD45R/B220 (RA3-6B2) PE
  • B_8F4_APC_012110
    C57BL/6 splenocytes stained with mouse IgG1 APC isotype control and CD45R/B220 (RA3-6B2) PE
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134809 25 µg 102 CHF
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134810 100 µg 343 CHF
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Description

B and T lymphocyte attenuator (BTLA) is an Ig superfamily coinhibitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4. BTLA is expressed on B cells, T cells, macrophages, dendritic cells, NKT cells, and NK cells. Engagement of BTLA by its ligand Herpes Virus Entry Mediator (HVEM) is critical for negatively regulating immune response. The absence of BTLA with HVEM inhibitory interactions leads to increased experimental autoimmune encephalomyelitis severity, enhanced rejection of partially mismatched allografts, an increased CD8+ memory T cell population, increased severity of colitis, reduced effectiveness of T regulatory cells. BTLA takes an important role in the induction of peripheral tolerance of both CD4+ and CD8+ T cells in vivo. Tolerant T cells have significant up-regulated expression of BTLA compared with effector and naïve T cells. BTLA may cooperate with CTLA-4 and PD-1 to control T cell tolerance and autoimmunity. It was reported that BTLA may regulate T cell function by binding to B7-H4. But further studies are needed to confirm. The existence of three distinct BTLA alleles was reported. The BTLA antibody reacts with mouse BTLA from both BALb/c and C57BL/6 strains.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C57BL/6 BTLA Ig domain protein in CFA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Hurchla MA, et al. 2005. J. Immunol. 174:3377
RRID
AB_2067835 (BioLegend Cat. No. 134809)
AB_2067835 (BioLegend Cat. No. 134810)

Antigen Details

Structure
An Ig superfamily coinhitory receptor with structural similarity to programmed cell death 1 (PD-1) and CTLA-4.
Distribution
BTLA is expressed on a wide number of lymphocytes in mice. It is most highly expressed on B cells, followed by CD4+T cells, lower express on CD8+ T cells, macrophages, dendritic cells, NKT cells, and NK cells.
Function
BTLA functions as a negative regulator of T cell activation and proliferation. It attenuates B cell proliferation upon associating with its known ligand, Herpes Virus Entry Mediator (HVEM).
Ligand/Receptor
Herpes Virus Entry Mediator (HVEM)
Cell Type
Lymphocytes, B cells, T cells, Dendritic cells, NKT cells
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Liu X, et al. 2009. J. Immunol. 182:4516
2. Miller ML, et al. 2009. J. Immunol. 183:32
3. Sun Y, et al. 2009. J. Immunol. 183:1946
4. Vendel AC, et al. 2009. J. Immunol. 182:1509
5. Watanabe N, et al. 2003. Nat. Immunol. 4(7):670
6. Sedy JR, et al. 2005. Nat Immunol. 6(1):90

Gene ID
208154 View all products for this Gene ID
UniProt
View information about CD272 on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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