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APC anti-mouse CD33 Antibody

Pricing & Availability
Clone
W18124D (See other available formats)
Regulatory Status
RUO
Other Names
sialic acid binding Ig-like lectin 3, SIGLEC3, SIGLEC-3, gp67, p67
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
W18124D_APC_CD33_Antibody_FC_101822
BALB/c mice splenocytes were stained with anti-mouse Ly-6G PE and anti-mouse CD33 (clone W18124D) APC (left) or rat IgG2a, λ PE isotype control (right).
  • W18124D_APC_CD33_Antibody_FC_101822
    BALB/c mice splenocytes were stained with anti-mouse Ly-6G PE and anti-mouse CD33 (clone W18124D) APC (left) or rat IgG2a, λ PE isotype control (right).
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104805 25 µg 144 CHF
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104806 100 µg 336 CHF
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Description

CD33, also known as Siglec-3, Sigle, gp67, p67, and gp6, is a member of the sialic acid-binding immunoglobulin lectins (Siglec). CD33 extracellular portion contains two immunoglobulin domains (one IgV and one IgC2 domain). The intracellular portion of CD33 contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that are implicated in inhibition of cellular activity. CD33 is primarily expressed on granulocytes in peripheral blood and spleen, as well as on myeloid precursors in the bone marrow.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
RBL1 Transfected with full length mCD33
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
RRID
AB_2927955 (BioLegend Cat. No. 104805)
AB_2927955 (BioLegend Cat. No. 104806)

Antigen Details

Structure
Extracellular portion contains two immunoglobulin domains (IgV and IgC2 domain), intracellular portion contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs)
Distribution

Granulocytes in peripheral blood and spleen, myeloid precursors in the bone marrow

Function
Inhibition of cellular activity
Ligand/Receptor
Molecule with sialic acid residues
Cell Type
Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Siglec Molecules
Antigen References
  1. Brinkman-Van der Linden, Els C M, et al. 2003. Mol Cell Biol. 23:4199-206.
Gene ID
12489 View all products for this Gene ID
UniProt
View information about CD33 on UniProt.org
Go To Top Version: 1    Revision Date: 10.18.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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