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APC anti-mouse CD63 Antibody

Pricing & Availability
Clone
NVG-2 (See other available formats)
Regulatory Status
RUO
Other Names
LIMP, LAMP-3, gp55, melanoma-associated antigen, ME491
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
NVG-2_APC_CD63_Antibody_1_040620
Thioglycolate-elicited BALB/c mouse peritoneal macrophages were fixed, permeabilized and intracellularly stained with CD11b PE and CD63 (clone NVG-2) APC (left) or rat IgG2a, κ APC isotype control (right).
  • NVG-2_APC_CD63_Antibody_1_040620
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages were fixed, permeabilized and intracellularly stained with CD11b PE and CD63 (clone NVG-2) APC (left) or rat IgG2a, κ APC isotype control (right).
  • NVG-2_APC_CD63_Antibody_FC_031115
    bEND.3 cells were stained with CD63 (clone NVG-2) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
Compare all formats See APC spectral data
Cat # Size Price Quantity Check Availability Save
143905 25 µg 137 CHF
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143906 100 µg 314 CHF
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Description

CD63, also known as LIMP, LAMP-3, gp55, and melanoma-associated antigen (ME491), is a member of the tetraspanin superfamily (TM4SF) that constitutes a main component of the lysosomal membrane. It is expressed on activated platelets, monocyte/macrophages, endothelium, fibroblasts, osteoblasts, and smooth muscle cells. CD63 may be involved in platelet activation and is thought to function as a transmembrane adaptor protein. CD63 has been shown to associate with CD9, CD81, VLA-3, and VLA-6. In mice, there are two CD63 gene loci, of which only one is functional. CD63 deficient mice are viable, and there is no alteration in the population of immune cells.  A recent report shows that CD63-deficient mice exhibit a significant reduction in both leukocyte rolling and recruitment in a peritonitis model. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Intestinal lamina propria light-density cells (enriched with eosinophils)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1 and immunofluorescence1.

Application References

(PubMed link indicates BioLegend citation)
  1. Verjan Garcia N, et al. 2011. J. Immunol. 187:2268. (WB, IF)
Product Citations
  1. McPherson HR, et al. 2021. Elife. 10:. PubMed
  2. Jiao Q, et al. 2020. Front Immunol. 11:469. PubMed
  3. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  4. Ratitong B, et al. 2021. Cell Reports. 35(7):109139. PubMed
  5. López-Sanz C, et al. 2022. STAR Protoc. 3:101755. PubMed
  6. Sanz-Ros J, et al. 2022. Sci Adv. 8:eabq2226. PubMed
  7. Lv L, et al. 2021. Front Immunol. 12:675534. PubMed
  8. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
RRID
AB_2565495 (BioLegend Cat. No. 143905)
AB_2565495 (BioLegend Cat. No. 143906)

Antigen Details

Structure
Tetraspan transmembrane superfamily (TM4SF), type III lysosomal glycoprotein, 53 kD
Distribution

Activated platelets, monocytes, macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle cells

Function
Platelet activation
Interaction
CD9, CD81, VLA-3, and VLA-6
Cell Type
Endothelial cells, Fibroblasts, Macrophages, Monocytes, Osteoclasts, Platelets
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Azorsa DO, et al. 1991. Blood 78:280.
2. Kishimoto T, et al. 1997. Leukocyte Typing V1. Oxford University Press New York.
3. Hildreth JE, et al. 1991. Blood 77:121.

Gene ID
12512 View all products for this Gene ID
UniProt
View information about CD63 on UniProt.org
Go To Top Version: 2    Revision Date: 04.06.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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