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APC/Cyanine7 anti-mouse CD117 (c-kit) Antibody

Pricing & Availability
Clone
2B8 (See other available formats)
Regulatory Status
RUO
Other Names
c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype
Rat IgG2b, κ
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Product Citations
publications
2B8_APCCyanine7_CD117_Antibody_022519
C57BL/6 mouse bone marrow cells were stained with CD11b FITC and CD117 (c-kit) (clone 2B8) APC/Cyanine7 (left) or rat IgG2b, κ APC/Cyanine7 isotype control (right).
  • 2B8_APCCyanine7_CD117_Antibody_022519
    C57BL/6 mouse bone marrow cells were stained with CD11b FITC and CD117 (c-kit) (clone 2B8) APC/Cyanine7 (left) or rat IgG2b, κ APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
105825 25 µg 94 CHF
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105826 100 µg 229 CHF
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Description

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse bone marrow mast cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.

Application References

(PubMed link indicates BioLegend citation)
  1. Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
  2. Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
  3. Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
  4. Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  2. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  3. Pohlmeier L, et al. 2021. Allergy. 76:2030. PubMed
  4. Kfoury YS, et al. 2021. Cell Stem Cell. 28:2090. PubMed
  5. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  6. Martin-Martin I, et al. 2022. Cell Rep. 39:110648. PubMed
  7. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
  8. Roy IM, et al. 2022. iScience. 25:105171. PubMed
  9. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  10. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  11. Ramdas B, et al. 2022. Mol Ther. 30:2505. PubMed
  12. Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed
  13. Gómez-Salinero JM, et al. 2022. Nat Cardiovasc Res. 1:882. PubMed
  14. Sambandam A, et al. 2023. Heliyon. 9:e14238. PubMed
  15. Herrejon Chavez F, et al. 2023. Nat Commun. 14:2290. PubMed
  16. Kang YA, et al. 2023. J Exp Med. 220:. PubMed
  17. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  18. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  19. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  20. Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
  21. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  22. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
  23. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  24. Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed
  25. Iturri L, et al. 2021. Immunity. . PubMed
  26. Wu X, et al. 2016. Nat Commun. 7:10533. PubMed
  27. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  28. Wu HC, et al. 2021. Cancer Discov. Online ahead of print. PubMed
  29. Li Z, et al. 2022. Elife. 11:. PubMed
  30. Taylor D, et al. 2011. J Immunol. 186:5956. PubMed
  31. Nguyen DTT, et al. 2020. Nat Commun. 11:2026. PubMed
  32. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  33. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  34. Khoramian Tusi B, et al. 2018. Exp Hematol. 60:21. PubMed
  35. Nurkhametova D, et al. 2019. Front Cell Neurosci. 13:45. PubMed
  36. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  37. Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed
  38. Li Y, et al. 2021. Nat Commun. 0.843055556. PubMed
  39. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  40. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  41. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  42. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  43. Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
  44. Bell CC, et al. 2019. Nat Commun. 10:2723. PubMed
  45. Reeh K, et al. 2014. Development. 141:2950. PubMed
  46. Beyaz S, et al. 2016. Nature. 531:53-58. PubMed
  47. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  48. Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
  49. Elahi S, et al. 2020. Stem Cell Res. 43:101710. PubMed
  50. Carr M, et al. 2016. Proc Natl Acad Sci U S A. 113(52):15024-15029. PubMed
  51. Comoglio F, et al. 2018. Genome Res. 10.1101/gr.227272.117. PubMed
  52. Cheng Y, et al. 2019. Cell Rep. 28:1703. PubMed
  53. Ku C, et al. 2015. Genes Dev. 29: 1930-1941. PubMed
  54. Minuesa G, et al. 2019. Nat Commun. 10:2691. PubMed
  55. Sevin M, et al. 2018. Nat Commun. 9:1431. PubMed
  56. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
  57. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  58. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  59. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  60. Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
  61. Cazares O, et al. 2021. Development. 148:. PubMed
  62. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  63. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  64. Mana MD, et al. 2021. Cell Reports. 35(10):109212. PubMed
  65. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  66. Abdala-Valencia H, et al. 2015. J Immunol. 195: 1377-1387. PubMed
  67. Rüder C, et al. 2014. PLoS One. 9:104644. PubMed
  68. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  69. Wang S, et al. 2021. Commun Biol. 22:. PubMed
  70. Stine RR, et al. 2020. Cell Stem Cell. 25(6):830-845. PubMed
  71. Bikorimana E, et al. 2014. J Vis Exp. 92: 52022. PubMed
  72. Shigeta A, et al. 2019. Dev Cell. 48:617. PubMed
  73. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  74. Koide S, et al. 2022. iScience. 25:103603. PubMed
  75. Kassas–Guediri A, et al. 2017. PLoS One. 12:e0173815. PubMed
  76. Krivtsov AV, et al. 2020. Cancer Cell. 36(6):660-673.e11.. PubMed
  77. Ortega-Molina A, et al. 2021. Cell Reports. 36(2):109372. PubMed
  78. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  79. Woods B, et al. 2019. Clin Cancer Res. 25:5901. PubMed
  80. Fransén Pettersson N, et al. 2018. PLoS One. 13:e0203228. PubMed
  81. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  82. Ito K et al. 2019. Cell Rep. 28(10):2480-2490 . PubMed
  83. Li X, et al. 2019. Stem Cells. 37:937. PubMed
  84. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  85. Lee SG, et al. 2020. J Nucl Med. . PubMed
  86. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
  87. Morganti C, et al. 2022. EMBO Rep. 23:e54262. PubMed
  88. Tu Z, et al. 2022. Cell Rep. 40:111072. PubMed
  89. Chavez JS, et al. 2022. Cells. 11:. PubMed
  90. Fielding C, et al. 2022. Nat Commun. 13:543. PubMed
  91. Park SM et al. 2018. Cell stem cell. 24(1):153-165 . PubMed
  92. Chaudhury S, et al. 2018. Nat Commun. 9:5280. PubMed
  93. Kang YA, et al. 2020. J Exp Med. 217:00:00. PubMed
  94. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
  95. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  96. Jee D et al. 2018. Molecular cell. 69(2):265-278 . PubMed
  97. Dingler FA, et al. 2020. Mol Cell. . PubMed
  98. Young K, et al. 2021. Cell Stem Cell. . PubMed
  99. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
RRID
AB_1626278 (BioLegend Cat. No. 105825)
AB_1626278 (BioLegend Cat. No. 105826)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Hematopoietic stem cells, AML, mast cells

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Stem Cell Factor (SCF)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.

Gene ID
16590 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD117 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD117 (c-Kit) 2B8 FC
Biotin anti-mouse CD117 (c-Kit) 2B8 FC
FITC anti-mouse CD117 (c-Kit) 2B8 FC
PE anti-mouse CD117 (c-Kit) 2B8 FC
PE/Cyanine5 anti-mouse CD117 (c-Kit) 2B8 FC
Purified anti-mouse CD117 (c-Kit) 2B8 FC,IHC-F
PE/Cyanine7 anti-mouse CD117 (c-Kit) 2B8 FC
Alexa Fluor® 488 anti-mouse CD117 (c-Kit) 2B8 FC,IHC-F
Alexa Fluor® 647 anti-mouse CD117 (c-Kit) 2B8 FC,IHC-F
Pacific Blue™ anti-mouse CD117 (c-Kit) 2B8 FC
PerCP/Cyanine5.5 anti-mouse CD117 (c-kit) 2B8 FC
PerCP anti-mouse CD117 (c-kit) 2B8 FC
APC/Cyanine7 anti-mouse CD117 (c-kit) 2B8 FC
Brilliant Violet 421™ anti-mouse CD117 (c-Kit) 2B8 FC,SB
Purified anti-mouse CD117 (c-Kit) (Maxpar® Ready) 2B8 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD117 (c-Kit) 2B8 FC
Brilliant Violet 711™ anti-mouse CD117 (c-Kit) 2B8 FC
Alexa Fluor® 594 anti-mouse CD117 (c-Kit) 2B8 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse CD117 (c-Kit) 2B8 FC
Brilliant Violet 510™ anti-mouse CD117 (c-Kit) 2B8 FC
Brilliant Violet 785™ anti-mouse CD117 (c-Kit) 2B8 FC
TotalSeq™-A0012 anti-mouse CD117 (c-kit) 2B8 PG
Brilliant Violet 605™ anti-mouse CD117 (c-Kit) 2B8 FC
Alexa Fluor® 700 anti-mouse CD117 (c-Kit) 2B8 FC
TotalSeq™-B0012 anti-mouse CD117 (c-Kit) 2B8 PG
TotalSeq™-C0012 anti-mouse CD117 (c-Kit) 2B8 PG
Spark NIR™ 685 anti-mouse CD117 (c-kit) 2B8 FC
Brilliant Violet 650™ anti-mouse CD117 (c-kit) 2B8 FC
Spark Red™ 718 anti-mouse CD117 (c-kit) (Flexi-Fluor™) 2B8 FC
Spark Blue™ 574 anti-mouse CD117 (c-kit) (Flexi-Fluor™) 2B8 FC
Spark Blue™ 550 anti-mouse CD117 (c-kit) (Flexi-Fluor™) 2B8 FC
Go To Top Version: 3    Revision Date: 02.25.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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