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APC/Cyanine7 anti-mouse Ly-6A/E (Sca-1) Antibody

Pricing & Availability
Clone
D7 (See other available formats)
Regulatory Status
RUO
Other Names
Sca-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
D7_APCCy7_072210
C57BL/6 splenocytes stained with D7 APC/Cyanine7
  • D7_APCCy7_072210
    C57BL/6 splenocytes stained with D7 APC/Cyanine7
Compare all formats See APC/Cyanine7 spectral data
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108125 25 µg 101 CHF
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108126 100 µg 311 CHF
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Description

Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent mouse T-cell line (CTL-L)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed, paraffin-embedded samples9.

The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14 

Application References

(PubMed link indicates BioLegend citation)
  1. Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP)
  2. Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB)
  3. Codias EK, et al. 1990. J. Immunol. 144:2197.
  4. Malek TR, et al. 1986. J. Exp. Med. 164:709.
  5. Codias EK, et al. 1990. J. Immunol. 145:1407.
  6. Ivanov V, et al. 1994. J. Immunol. 153:2394.
  7. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  8. Fleming T, et al. 1994. J. Immunol. 153:1955.
  9. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC)
  10. Umland O, et al. 2007. J. Immunol. 178:4147.
  11. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed
  12. Pronk CJ, et al. 2011. J. Exp Med. PubMed
  13. English A, et al. 2000. J. Immunol. 165:3763. (IHC)
  14. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
  15. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed
Product Citations
  1. Barros-Silva JD, et al. 2018. Cell Rep. 25:3504. PubMed
  2. Abraham A, et al. 2019. J Clin Invest. 130:2685. PubMed
  3. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  4. Chakraborty P, et al. 2022. Cancer Res. 82:1969. PubMed
  5. McAlpine CS, et al. 2022. J Exp Med. 219: . PubMed
  6. Xin DQ, et al. 2022. Neural Regen Res. 17:2238. PubMed
  7. Schönberger K, et al. 2023. Anal Chem. 95:4325. PubMed
  8. Kurbangaleeva S, et al. 2023. Curr Issues Mol Biol. 45:2431. PubMed
  9. Gautheron F, et al. 2023. Cell Death Discov. 9:117. PubMed
  10. Lütge M, et al. 2023. Nat Immunol. . PubMed
  11. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  12. Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed
  13. Corna G, et al. 2016. J Immunol. 197: 1914 - 1925. PubMed
  14. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  15. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  16. Larsen SB, et al. 2021. Cell Stem Cell. 28:1758. PubMed
  17. Chen X, et al. 2021. Theranostics. 11:4655. PubMed
  18. Nawaz A, et al. 2022. Nat Commun. 13:7058. PubMed
  19. Fu X, et al. 2015. J Biol Chem. 290: 26445 - 26456. PubMed
  20. Valds-Mora F, et al. 2021. Cell Reports. 35(2):108945. PubMed
  21. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  22. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  23. Joshi PA, et al. 2019. Nat Commun. 10:1760. PubMed
  24. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  25. Jakob L, et al. 2021. Int J Mol Sci. 22:. PubMed
  26. McAlpine CS, et al. 2021. Nature. 595:701. PubMed
  27. Grandl G, et al. 2016. Mol Metab. 5:937-947. PubMed
  28. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  29. Yang SH, et al. 2017. Front Immunol. 8:1192. PubMed
  30. Gomzikova MO, et al. 2020. Sci Rep. 10:10740. PubMed
  31. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
  32. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  33. Carr M, et al. 2016. Proc Natl Acad Sci U S A. 113(52):15024-15029. PubMed
  34. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  35. Helbling PM, et al. 2019. Cell Rep. 29:3313. PubMed
  36. Hermetet F, et al. 2019. Nat Commun. 10:523. PubMed
  37. Agarwal P, et al. 2019. Cell Stem Cell. 24:769. PubMed
  38. Ye H, et al. 2021. Cancer Discov. 0.805555556. PubMed
  39. Koyama T, et al. 2022. Curr Issues Mol Biol. 44:3146. PubMed
  40. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  41. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  42. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  43. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  44. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  45. Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
  46. Ozaki M, et al. 2021. J Stem Cells Regen Med. 16:50. PubMed
  47. Jie Z, et al. 2014. J Immunol. 192:3289. PubMed
  48. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
  49. Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed
  50. Gross KM, et al. 2019. Cell Rep. 28:394. PubMed
  51. Hu B, et al. 2020. J Clin Invest. 130:3483. PubMed
  52. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  53. Agarwal P, et al. 2021. Cell Reports. 36(2):109386. PubMed
  54. Jatho A, et al. 2022. Cells. 11:. PubMed
  55. Fast EM, et al. 2021. Elife. 10:. PubMed
RRID
AB_10645327 (BioLegend Cat. No. 108125)
AB_10645327 (BioLegend Cat. No. 108126)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution

Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells

Function
Regulates B and T cell responses
Cell Type
B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org
Go To Top Version: 3    Revision Date: 07.11.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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