APC/Fire™ 750 anti-human CD25 Antibody, CD25, M-A251

Pricing & Availability

Analyte Specific Reagent. Analytical and performance characteristics are not established.

Clone: M-A251
Workshop: IV A053
Other Names: IL-2 receptor α chain, Low affinity IL-2R, IL-2Rα chain
Isotype: Mouse IgG1, κ

Typical results from human peripheral blood lymphocytes stained either with M-A251 APC/Fire™ 750 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).

Compare all formats See APC/Fire™ 750 spectral data

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985806

500 µL

376 CHF

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Description

CD25 is a 55 kD type I transmembrane glycoprotein also known as low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4⁺ T cells termed T regulatory cells. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses. CD25 associates with IL-2 receptor β (CD122) and common γ (CD132) chains to form a high affinity IL-2R complex.

Product Details

Technical Data Sheet (pdf)

Product Details

Reactivity: Human
Formulation: Phosphate-buffered solution, pH7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide, 0.2% (w/v) BSA (origin USA), and a stabilizer.
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration: 100 µg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: Suggested for Flow Cytometry

Antigen Details

Antigen References: 1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
3. Barclay N, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press Inc.
4. Taniguchi T and Minami Y. et al. 1993. Cell 73:5.
5. Waldmann T. 1991. J. Biol. Chem. 266:2681.

Documentation

Certificate of Analysis

Go To Top Version: 2 Revision Date: 08.22.2024

Analyte Specific Reagent. Analytical and performance characteristics are not established.

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