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APC/Fire™ 750 anti-human CD52 Recombinant Antibody

Pricing & Availability
Clone
QA19A22 (See other available formats)
Regulatory Status
RUO
Other Names
Cambridge pathology antigen 1 (CAMPATH-1), epididymal secretory protein 1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
QA19A22_APCFire750_CD52_RECOM_Antibody_080321.png
Human peripheral blood lymphocytes were stained with anti-human CD52 recombinant (clone QA19A22) APC/Fire750™ (filled histogram) or mouse IgG2a, κ APC/Fire750™ isotype control (open histogram).
  • QA19A22_APCFire750_CD52_RECOM_Antibody_080321.png
    Human peripheral blood lymphocytes were stained with anti-human CD52 recombinant (clone QA19A22) APC/Fire750™ (filled histogram) or mouse IgG2a, κ APC/Fire750™ isotype control (open histogram).
Compare all formats See APC/Fire™ 750 spectral data
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318913 25 tests 147 CHF
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318914 100 tests 347 CHF
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Description

CD52, also known as Cambridge pathology antigen 1 (CAMPATH-1), is a 25-29 kD glycoprotein containing a large N-linked carbohydrate moiety. The actual molecule of CD52 is only 8-9 kD. It is expressed in the male reproductive tract and on virtually all lymphocytes (T and B cells), as well as macrophages/monocytes, eosinophils, and red cells. CD52 is thought to play a role in carrying and orienting carbohydrates. CD52 is a potent target for complement-mediated lysis and antibody-mediated cellular cytotoxicity and has been used as a depletion target for chronic lymphocytic leukemia (CLL)/lymphoma and immunosuppression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Excitation Laser
Red Laser (633 nm)
RRID
AB_2894466 (BioLegend Cat. No. 318913)
AB_2894466 (BioLegend Cat. No. 318914)

Antigen Details

Structure
Unusually small glycopeptide containing N-linked carbohydrate moiety, GPI-linked membrane protein, approximate molecular weight 7 kD
Distribution

Male reproductive tract (epididymis, seminal vesicle), T cells, B cells, macrophage/monocyte, eosinophil, erythrocytes, malignant lymphocytes

Function
Thought to play a role in carrying and orienting carbohydrates. Target for complement-mediated lysis and antibody-dependent cellular cytotoxicity, used as a depletion target for chronic lymphocytic leukemia/lymphoma therapy and immunosuppression.
Antigen References

1. Leukocyte Typing VI. Kishimoto T, et al. (Eds.) Garland Publishing Inc. (1997)
2. Xia MQ, et al. 1991. Eur. J. Immunol. 21:1677.
3. Kirchhoff C, et al. 1993. Mol. Reprod. Dev. 34:8.
4. Xia MQ, et al. 1993. Biochem. J. 293:633.

Gene ID
1043 View all products for this Gene ID
UniProt
View information about CD52 on UniProt.org
Go To Top Version: 1    Revision Date: 08.03.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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