APC Streptavidin

Product Details

Verified Reactivity

Human, Mouse, Rat, All Species

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

Streptavidin is conjugated with APC under optimal conditions.

Concentration

0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)

Storage & Handling

The Streptavidin-APC solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

ICFC - Verified

Recommended Usage

Each lot of this Streptavidin-APC is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the APC conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.125 µg per 10⁶ cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

Streptavidin-APC is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-APC is 320 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)

1. Kamimura D, et al. 2006. J. Immunol. 177:306.
2. Andoniou CE, et al. 2005. Nature Immunology 6:1011.
3. Chou WC,et al.2006.Blood108:3005. PubMed
4. Malagolini N, et al. 2009. Glycobiology. 19:172. PubMed
5. Kroeger KM, et al. 2009. J. Leukocyte Biol. PubMed
6. Imada M, et al. 2009. Int. Immunol. 21:1151. PubMed
7. Yi Y, et al. 2010 J. Biol Chem. 285:21233. PubMed
8. King IL, et al. 2010. J. Immunol. 185:6138. PubMed
9. Yokoyama T, et al. 2011. Int Immunol. PubMed
10. Rusert P, et al. 2011. J. Exp Med. 208:1419. PubMed
11. Bollig N, et al. 2012. PNAS. 109:8664. PubMed
12. Nguyen V, et al. 2012. J. Immunol. 189:1081. PubMed
13. Jager NA, et al. 2012. J Nucl Med. 53:1229. PubMed
14. Liberman AC, et al. 2012. Mol Immunol. 50:220. PubMed
15. Ogawa H, et al. 2012. Biochem Biophys Res Commun. 420:114. PubMed
16. Ludin A, et al.2012. Nat Immunol. 13:1072. PubMed
17. Sugiura D, et al. 2012. PLoS One. 7:e44770. PubMed
18. Tani-Ichi S, et al. 2012. PNAS. 110:612. PubMed
19. Jin L, et al. 2013. PNAS. 110:3907. PubMed
20. Ota T, et al. 2013. J. Immunol. 191:3179. PubMed
21. Fu X, et al. 2013. PNAS. 110:17982. PubMed
22. Ryan SO, et al. 2014. Glycobiology. 24:362. PubMed
23. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
24. Pilling D, et al. 2014. PLoS One. 9:93730. PubMed
25. Matthews JA, et al. 2014. PLoS One. 9:97707. PubMed
26. Owen KA, et al. 2014. PLoS Pathog. 10:1004159. PubMed
27. Duek A, et al. 2014. Blood. 123:3943. PubMed
28. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
29. Skogberg G, et al. 2014. J Immunol. 193:2187. PubMed
30. Lundberg P, et al. 2014. J Exp Med. PubMed
31. Martin RK, et al. 2014. PLoS One. 9:110609. PubMed
32. Rai S, et al. 2014. PLoS One. 9:109441. PubMed
33. Santoyo-Ramos P, et al. 2014. PLoS One. 9:112580. PubMed
34. Jager NA, et al. 2014. J Nucl Med. 55:1945. PubMed
35. Kong S, et al. 2014. J Immunol. 193:5515. PubMed
36. Cabrera-Perez J, et al. 2015. J Immunol. 194:1609. PubMed
37. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
38. Kim Yu, et al. 2015. PLoS One. 10:120294. PubMed
39. Zhang L, et al. 2015. J Immunol. 194:5243. PubMed

See More

Product Citations

1. Goel RR, et al. 2022. Cell. 185:1875. PubMed
2. Sugimoto M, et al. 2022. DNA Res. 29: . PubMed
3. Nutalai R, et al. 2022. Cell. 185:2116. PubMed
4. Singh S, et al. 2022. iScience. 25:104549. PubMed
5. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
6. Scharf L, et al. 2023. JCI Insight. 8: . PubMed
7. Cao Y, et al. 2023. Nature. 614:521. PubMed
8. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
9. Hernandez SPA, et al. 2023. Front Immunol. 13:1035344. PubMed
10. Pušnik J, et al. 2023. Nat Commun. 14:572. PubMed
11. Gon Y, et al. 2023. PLoS One. 18:e0279389. PubMed
12. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
13. Dickerson KM, et al. 2022. Blood Cancer Discov. 3:240. PubMed
14. Lopez R, et al. 2022. Nat Biotechnol. 40:1360. PubMed
15. White Z, et al. 2022. Blood Cells Mol Dis. 97:102688. PubMed
16. Chen HY, et al. 2022. Elife. 11:. PubMed
17. Parry H, et al. 2022. Cell Rep Med. 3:100739. PubMed
18. Vyasamneni R, et al. 2023. Cell Rep Methods. 3:100388. PubMed
19. Nihei Y, et al. 2023. Sci Adv. 9:eadd6734. PubMed
20. Koutsakos M, et al. 2023. Immunity. 56:879. PubMed
21. García-Vega M, et al. 2023. iScience. 26:106562. PubMed
22. Liu Y, et al. 2023. Nat Commun. 14:2179. PubMed
23. Hie B, et al. 2023. Nat Biotechnol. . PubMed
24. Li X, et al. 2023. Nat Commun. 14:2929. PubMed
25. Lan Y, et al. 2023. iScience. 26:106817. PubMed
26. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
27. Suzuki M, et al. 2015. J Immunol. 195: 1273-1281. PubMed
28. Preece R, et al. 2020. Gene Ther. 451:27. PubMed
29. Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
30. Perruzza L, et al. 2019. Sci Rep. 9:9315. PubMed
31. Cohen–Khait R, et al. 2017. Structure. 25:1867. PubMed
32. Tarban N, et al. 2022. Cells. 11:. PubMed
33. Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
34. Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed
35. Georgiadis C, et al. 2021. Leukemia. . PubMed
36. Malagolini N, et al. 2009. Glycobiology. 0.911111111. PubMed
37. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
38. Schneider C, et al. 2018. Cell. 174:271. PubMed
39. Proietti M, et al. 2019. Nat Commun. 10:250. PubMed
40. Temming AR, et al. 2021. Front Immunol. 12:594773. PubMed
41. Blake SJ, et al. 2021. Cell Rep Med. 2:100464. PubMed
42. Sarkar R, et al. 2021. Bio Protoc. 11:e4255. PubMed
43. Hou S, et al. 2021. Sci Immunol. 6:eabg9433. PubMed
44. Ota T, et al. 2013. J Immunol. 191:3179. PubMed
45. Santoyo-Ramos P, et al. 2014. PLoS One. 9:112580. PubMed
46. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
47. Viborg N, et al. 2019. Oncoimmunology. 8:e1663107. PubMed
48. Pazina T, et al. 2019. Cancer Immunol Res. 7:1633. PubMed
49. da Mota JB, et al. 2020. J Leukoc Biol. 108:1239. PubMed
50. Niewolik D, et al. 2022. Nucleic Acids Res. 50:2096. PubMed
51. Io S, et al. 2021. STAR Protoc. 2:100921. PubMed
52. Yamanaka S, et al. 2021. STAR Protocols. 2(1):100314. PubMed
53. J L, et al. 2016. Sci Rep. 6:30904. PubMed
54. Gaudreault V, et al. 2015. PLoS One. 10: e0140805. PubMed
55. Jager N, et al. 2014. J Nucl Med. 55:1945. PubMed
56. Ludin A, et al. 2012. Nat Immunol. 13:1072. PubMed
57. Jager N, et al. 2012. J Nucl Med. 53:1222. PubMed
58. Kimura T, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 317:L271. PubMed
59. Biffi G, et al. 2018. Cancer Discov. 2:282. PubMed
60. Lebert-Ghali C, et al. 2017. Exp Hematol. 10.1016/j.exphem.2017.03.003. PubMed
61. Mathews J, et al. 2014. PLoS One. 9:97707. PubMed
62. Kim Y, et al. 2015. PLoS One. 10:120294. PubMed
63. Jin L, et al. 2016. Stem Cell Res. 16:40-53. PubMed
64. Palm A, et al. 2016. Sci Rep. 10.1038/srep20531. PubMed
65. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
66. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
67. Catapano M, et al. 2020. J Invest Dermatol. 140:816. PubMed
68. Rusert P, et al. 2011. J Exp Med. 208:1419. PubMed
69. Liu C, et al. 2022. Cell Host Microbe. 30:53. PubMed
70. She L, et al. 2021. JCI Insight. 6:e143509. PubMed
71. Cao Y, et al. 2022. Nature. :. PubMed
72. Sato Y, et al. 2021. J Clin Invest. Online ahead of print. PubMed
73. Toulmin SA, et al. 2021. Nat Commun. 12:3993. PubMed
74. Barcala Tabarrozzi A, et al. 2016. Sci Rep. 6:36646. PubMed
75. Kong S, et al. 2014. J Immunol. 193:5515. PubMed
76. Gui J, et al. 2020. Nat Cancer. 1:603. PubMed
77. Duek A, et al. 2014. Blood. 123:3943. PubMed
78. Yokoyama T, et al. 2011. J Immunol. 208:885. PubMed
79. Cao Y, et al. 2022. Nature. 602:657. PubMed
80. Kakizaki M, et al. 2021. Cell Death Dis. 12:1010. PubMed
81. Calés C, et al. 2016. Mol Cell Biol. 36: 900 - 912. PubMed
82. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
83. Bollig N, et al. 2012. Proc Natl Acad Sci U S A. 109:8664. PubMed
84. Yi Y, et al. 2010. J Biol Chem. 285:21233. PubMed
85. Chou W, et al. 2006. Blood. 108:3005. PubMed
86. Tashita C, et al. 2020. World J Gastroenterol. 26:918. PubMed
87. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
88. Wang L, et al. 2022. Nat Commun. 13:4709. PubMed
89. Kunitomi A, et al. 2022. Cell Rep Methods. 2:100317. PubMed
90. Tani-ichi S, et al. 2012. Proc Natl Acad Sci U S A. 110:612. PubMed
91. Ryan S, et al. 2014. Glycobiology. 24:262. PubMed
92. Han X, et al. 2017. Mol Ther. 25(1):274-284. PubMed
93. Hao Y, et al. 2020. J Biol Chem. . PubMed
94. Parikh BA, et al. 2020. Cell Rep. 32:107969. PubMed
95. Hansen UK, et al. 2020. Front Immunol. 11:373. PubMed
96. Mizoguchi I, et al. 2020. J Clin Invest. 130:6124. PubMed
97. Locke F, et al. 2017. Mol Ther. 25(1):285-295. PubMed
98. Jennings R, et al. 2016. Int Immunol. 28: 503 - 512. PubMed
99. Morita N, et al. 2016. Innate Immunity. 10.1177/1753425916672312. PubMed
100. Rai S, et al. 2014. PLoS One. 9:109441. PubMed
101. Owen K, et al. 2014. PLoS Pathog. 10:1004159. PubMed
102. Sugiura D, et al. 2012. PLoS One. 7:e44770. PubMed
103. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
104. Friedrich SK, et al. 2021. Pathogens. 10: . PubMed
105. Grioni M, et al. 2021. Blood Adv. 5:2817. PubMed
106. Nguyen V, et al. 2012. J Immunol. 189: 1081. PubMed
107. Jin L, et al. 2013. Proc Natl Acad Sci U S A. 110:3907. PubMed
108. James KR, et al. 2020. Nat Immunol. 1.113194444. PubMed
109. Audemard-Verger A, et al. 2017. J Immunol. 10.4049/jimmunol.1700430. PubMed
110. Hermann BP, et al. 2018. Cell Rep. 25:1650. PubMed
111. Van Gool F, et al. 2019. Immunity. 50:362. PubMed
112. Pascual G, et al. 2021. Nature. 599:485. PubMed
113. Saini SK, et al. 2021. Sci Immunol. 6:. PubMed
114. Mainini F, et al. 2015. PLoS One. 10: 0145403. PubMed
115. Zhang L, et al. 2015. J Immunol. 194:5423. PubMed
116. Skogberg G, et al. 2014. J Immunol. 193:2187. PubMed
117. Ogawa H, et al. 2012. Biochem Biophys Res Commun. 420:114. PubMed
118. Delacher M, et al. 2020. Immunity. 52:295. PubMed
119. Guichard V, et al. 2017. Elife. 6:e27215. PubMed
120. Collins DR, et al. 2021. Immunity. 54:2372. PubMed
121. Larcombe-Young D, et al. 2022. STAR Protoc. 3:101414. PubMed
122. Pilling D, Gomer R 2014. PLoS One. 9:93730. PubMed
123. Dejnirattisai W, et al. 2021. Cell. 2183:184. PubMed
124. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
125. Jiang H, et al. 2011. PLoS One. 6:e27464. PubMed
126. Tuekprakhon A, et al. 2022. Cell. 185:2422. PubMed
127. Kim SP, et al. 2022. Cancer Immunol Res. :OF1. PubMed
128. Robertson TF, et al. 2021. J Cell Biol. 220:. PubMed
129. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
130. Lundberg P, et al. 2014. J Exp Med. 211:2213. PubMed
131. King I, et al. 2010. J Immunol. 185:6138. PubMed
132. Budai Z, et al. 2021. Cells. 10:. PubMed
133. Bainter W, et al. 2021. Sci Immunol. 6:eabf6723. PubMed
134. Martin R, et al. 2014. PLoS One. 9:110609. PubMed
135. Gerdes P, et al. 2022. Nat Commun. 13:7470. PubMed
136. Koide S, et al. 2022. iScience. 25:103603. PubMed
137. Tian D, et al. 2019. Nat Commun. 10:4246. PubMed
138. Yewdell WT, et al. 2021. Cell Rep. 37:109961. PubMed
139. Nguyen NT, et al. 2021. Nat Nanotechnol. 16:1424. PubMed
140. Kosti P, et al. 2021. STAR Protocols. 2:100723. PubMed
141. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
142. Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed
143. Mazor Y, et al. 2017. Sci Rep. 7:40098. PubMed
144. Wan X, Thomas J, Unanue E 2016. J Exp Med. 213: 967 - 978. PubMed
145. Liberman A, et al. 2012. Mol Immunol. 50:220. PubMed
146. GR N, et al. 2012. PLoS One. 7:e39045. PubMed
147. Zahradník J, et al. 2021. ACS Synth Biol. Online ahead of print. PubMed
148. Chen Y, et al. 2021. Cancer Res Treat. Online ahead of print. PubMed
149. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
150. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
151. Tripathi S, et al. 2016. Proc Natl Acad Sci U S A. 113: 1555 - 1564. PubMed
152. Hauser J, et al. 2016. Mol Immunol. 80:78-90. PubMed
153. Wang W, et al. 2020. STAR Protoc. 1:100218. PubMed
154. Pedersen NW, et al. 2019. Nat Commun. 10:837. PubMed
155. Nagatake T, et al. 2019. Mucosal Immunol. 12:p1082. PubMed
156. Yang C, et al. 2019. Nat Commun. 10:3931. PubMed
157. Kroeger K, et al. 2009. J Leukoc Biol. 86:769. PubMed
158. Fichtner ML, et al. 2022. Acta Neuropathol Commun. 10:154. PubMed
159. Holm JS, et al. 2022. Nat Commun. 13:1935. PubMed
160. Spanier J, et al. 2016. Nat Commun. 7:11804. PubMed
161. Tagawa M, et al. 2016. PLoS One. 11: 0150030. PubMed
162. Fu X, et al. 2013. Proc Natl Acad Sci U S A. 110:17892. PubMed

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Antigen Details

Gene ID

NA

UniProt

View information about Biotin on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Certificate of Analysis