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APC/Fire™ 750 anti-human CD85j (ILT2) Antibody

Pricing & Availability
Clone
GHI/75 (See other available formats)
Regulatory Status
RUO
Workshop
V B032
Other Names
ILT2, LIR-1, LILRB1, CD85
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
GHIslash75_APCFire750_CD85j_Antibody_020918
Human peripheral blood lymphocytes were stained with CD85j (clone GHI/75) APC/Fire™ 750 (filled histogram) or mouse IgG2b, κ APC/Fire™ 750 isotype control (open histogram).
  • GHIslash75_APCFire750_CD85j_Antibody_020918
    Human peripheral blood lymphocytes were stained with CD85j (clone GHI/75) APC/Fire™ 750 (filled histogram) or mouse IgG2b, κ APC/Fire™ 750 isotype control (open histogram).
Compare all formats See APC/Fire™ 750 spectral data
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333717 25 tests 159 CHF
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333718 100 tests 364 CHF
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Description

CD85 is a group of Ig superfamily tansmembrane glycoproteins called Ig-Like Transcripts (ILTs) or Leukocyte Immunoglobulin-like Receptors (LIRs). CD85j is the 110kD member, known as ILT2, LIR1, or LILRB1, and MIR7. ILT2 structurally has four Ig domains and contains ITIMs in its cytoplasmic tail that provide inhibitory signals by recruiting SHP-1. ILT2 is found on the surface of B cells, plasma cells, dendritic cells, monocytes, subsets of NK and T cells. The ligands of ILT2 include a broad range of HLA-A, -B molecules, some HLA-C and HLA-G molecules, and the human cytomegalovirus UL18 protein.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported application: Block HLA-G induced TGF-ß1 production.

Application References

(PubMed link indicates BioLegend citation)
  1. Pulford K, et al. 1991. Clin. Exp. Immunol. 85:429
  2. Mclntire RH, et al. 2004. J. Leukoc. Biol. 76:1220
Product Citations
  1. Grutza R, et al. 2020. J Immunol. 204:2910. PubMed
RRID
AB_2728291 (BioLegend Cat. No. 333717)
AB_2728291 (BioLegend Cat. No. 333718)

Antigen Details

Structure
ILT/LIR family, Ig superfamily, 110 kD
Distribution

B cells, plasma cells, dendritic cells, monocytes, subsets of NK and T cells.

Function
Inhibitory receptor for MHC class I
Ligand/Receptor
HLA-A, -B, some HLA-C and G molecules, human cytomegalovirus UL18 protein
Cell Type
B cells, Dendritic cells, Monocytes, NK cells, Plasma cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication
2. Kirwan SE and Burshtyn DN. 2005. J. Immunol. 175:5006

Gene ID
10859 View all products for this Gene ID
UniProt
View information about CD85j on UniProt.org
Go To Top Version: 0    Revision Date: 03.19.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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