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APC/Fire™ 750 anti-mouse CD301 (MGL1/MGL2) Antibody

Pricing & Availability
Clone
LOM-14 (See other available formats)
Regulatory Status
RUO
Other Names
MGL, M-ASGP-BP, Macrophage galactose-type C-type lectin, CLEC10A, CD301a/CD301b
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
LOM-14_APCFire750_CD301_Antibody_1_120919
C57BL/6 bone marrow derived dendritic cells were stained with CD11c PE and CD301 (MGL1/MGL2) (clone LOM-14) APC/Fire™ 750 (left) or rat IgG2b, κ APC/Fire™ 750 isotype control (right).
  • LOM-14_APCFire750_CD301_Antibody_1_120919
    C57BL/6 bone marrow derived dendritic cells were stained with CD11c PE and CD301 (MGL1/MGL2) (clone LOM-14) APC/Fire™ 750 (left) or rat IgG2b, κ APC/Fire™ 750 isotype control (right).
Compare all formats See APC/Fire™ 750 spectral data
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145711 25 µg 147 CHF
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145712 100 µg 347 CHF
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Description

Mouse CD301, also known as macrophage galactose-type C-type lectin, has two homologue genes, CD301a (MGL1) and CD301b (MGL2), while there is only one MGL in human and rat. Mouse CD301a and CD301b are ~42 kD type II transmembrane glycoproteins containing a cytoplasmic domain, a transmembrane domain, a neck domain, and a carbohydrate recognition domain (CRD) within each molecule. CD301a is mainly expressed on a subset of macrophages and immature dendritic cells (DCs). CD301b is mainly found on conventional DCs. Although CD301a and CD301b share high amino acid sequence homology (92% for the intact sequence and 80% for the CRD), they display different carbohydrate specificities. CD301a was found to be highly specific for Lewis X and Lewis A structures, whereas CD301b, more similar to the human MGL, recognizes N-actetylgalactosamine (GalNAc) and galactose, including the O-linked Tn-antigen, TF-antigen, and core 2. So far, CD301a has been reported to be involved in recognition and  endocytosis of glycoproteins with terminal Gal/GalNAc moieties. This contributes to defense against tumor cell metastasis, tissue remodeling, and clearance of apoptotic cells in embryos. CD301b is involved in the internalization of soluble polyacrylamide polymers (PAA) with α-GalNAc residues (GalNAc-PAA) in bone marrow derived dendritic cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Purified and recombinant mouse MGL1 and MGL2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

LOM-14 recognizes mouse CD301a and CD301b. Additional reported applications (for relevant formats) include: immunohistochemical staining of frozen tissue sections1-3 and immunoprecipitation4.

Application References

(PubMed link indicates BioLegend citation)
  1. Denda-Nagai K, et al. 2010. J. Biol. Chem. 285:19193. (IHC)
  2. Sato K, et al. 2005. Blood 106:207. (IHC)
  3. Tsuiji M, et al. 2002. J. Biol. Chem. 277:28892. (IHC)
  4. Kimura T, et al. 1995. J. Biol. Chem. 270:16056. (IP)
RRID
AB_2819893 (BioLegend Cat. No. 145711)
AB_2819893 (BioLegend Cat. No. 145712)

Antigen Details

Structure
42 kD type II transmembrane glycoprotein, contain a cytoplasmic domain, a transmembrane domain, a neck domain, and a carbohydrate recognition domain (CRD)
Distribution

Immature dendritic cells, a subset of macrophages

Function
Cell adhesion, cell-cell signaling, glycoprotein turnover, inflammation, tissue remodeling
Interaction
Carbohydrate determinants, GP envelope glycoprotein on Marburg and Ebola viruses
Ligand/Receptor
Glycans containing terminal GalNAc moieties
Cell Type
Dendritic cells, Macrophages
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Denda-Nagai K, et al. 2010. J. Biol. Chem. 285:19193.
2. Westcott D, et al. 2009. J. Exp. Med. 206:3143.
3. Singh SK, et al. 2009. Mol. Immunol. 46:1240.
4. Sakakura M, et al. 2008. J. Biol. Chem. 283:33665.
5. Tsuiji M, et al. 2002. J. Biol. Chem. 277:28892.

Gene ID
17312 View all products for this Gene ID 216864 View all products for this Gene ID
UniProt
View information about CD301 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12.09.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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