APC/Fire™ 750 anti-mouse CD86 Antibody, CD86, PO3

Pricing & Availability

Clone: PO3 (See other available formats)
Regulatory Status: RUO
Other Names: B7-2, B70, Ly-58
Isotype: Rat IgG2b, κ
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Product Citations: publications

PO3_APC_F750_CD86_Antibody_121621 — LPS stimulated (3 days) C57BL/6J splenocytes were stained with anti-mouse CD86 (clone PO3) APC/Fire™ 750 (filled histogram) or rat IgG2b, κ APC/Fire™ 750 isotype control (open histogram).

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105129

25 µg

106 CHF

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105130

100 µg

293 CHF

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Description

CD86 is an 80 kD immunoglobulin superfamily member, also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells and astrocytes. CD86 along with CD80 are the ligands of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells. The PO3 antibody has been shown to block autoantibody production in vivo and inhibit T cell activation in vitro.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: BALB/c mouse B leukemia cell line BCL1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: in vivo and in vitro blocking ^1,4,5 of autoantibody production and T cell activation, stimulation of B cell activity³, immunoprecipitation², and immunohistochemical staining² of acetone-fixed frozen sections.
RRID: AB_3068031 (BioLegend Cat. No. 105129)
AB_3068031 (BioLegend Cat. No. 105130)

Antigen Details

Structure: Ig superfamily, 80 kD
Distribution: B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes
Function: T cell costimulation, Ig class-switching, NK cell cytotoxicity
Ligand/Receptor: CD28, CD152 (CTLA-4)
Cell Type: Astrocytes, B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science. 262:905.
3. Freeman GJ, et al. 1993. Science. 262:907.
4. Carreno BM, et al. 2002. Annu Rev Immunol. 20:29.
Gene ID: 12524 View all products for this Gene ID
UniProt: View information about CD86 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD86 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-mouse CD86	PO3	FC
Purified anti-mouse CD86	PO3	FC,IHC-F,IP
FITC anti-mouse CD86	PO3	FC
APC anti-mouse CD86	PO3	FC
PE/Cyanine7 anti-mouse CD86	PO3	FC
Alexa Fluor® 700 anti-mouse CD86	PO3	FC
Brilliant Violet 421™ anti-mouse CD86	PO3	FC
Brilliant Violet 605™ anti-mouse CD86	PO3	FC
PerCP/Cyanine5.5 anti-mouse CD86	PO3	FC
APC/Fire™ 750 anti-mouse CD86	PO3	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 12.16.2021

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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