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- Regulatory Status
- RUO
- Other Names
- Stromal cell-derived factor 1, chemokine (C-X-C motif) ligand 12, SCYB12, PBSF, SCYB12, Pre-B Cell Growth-Stimulating Factor, Chemokine (C-X-C Motif) Ligand 12
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- Product Citations
- publications
-
Human peripheral blood lymphocytes were surface stained with PE mouse IgG1, κ isotype control (Cat. No. 400113) (left panel), PE anti-human CD184 (CXCR4) Recombinant (Cat. No. 304503) (middle panel), or with AZDye 647 Recombinant Human CXCL12 (right panel). -
Biological activity was performed in HEK293T cells expressing human CXCR4 by measuring its ability to recruit β-arrestin2. The EC50 for this effect was calculated at 4 to 10 ng/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 598512 | 10 µg | 375 CHF | ||||
CXCL12, also known as SDF1, belongs to the CXCL chemokine family. SDF1 is expressed by many organs, and it is most abundantly expressed in pancreas, spleen, ovary, and small intestine. SDF1 and its receptor CXCR4 are involved in regulation of migration, survival, and development of multiple cell types, including human hematopoietic CD34+/CD38-/low and stromal STRO-1+ stem cells. Stress-induced modulations in SDF1 and CXCR4 levels participate in recruitment of immature and maturing leukocytes from the BM reservoir to damaged organs as part of host defense and repair mechanism. SDF1/CXCR4 system is involved in the establishment of organ metastasis in different cancers, for example in lymph node metastasis in breast cancer and oral squamous cell carcinoma (SCC), and peritoneal metastasis in ovarian cancer. Several studies have demonstrated the existence of a small subset of cancer cells which share many characteristics with stem cells and named cancer stem cells (CSC). They constitute a reservoir of self-sustaining cells with the ability to maintain the tumor growth. Most of them express CXCR4 receptor and respond to a chemotactic gradient of its specific ligand SDF1, suggesting that CSC probably represent a subpopulation capable of initiating metastasis.
Product DetailsProduct Details
- Source
- Human CXCL12, amino acids Lys22-Lys89 (Accession # P48061), with a C-terminal AZDye647, was expressed in E. coli.
- Molecular Mass
- The 93 amino acid recombinant protein has a predicted molecular weight mass of 9.9 kDa.
- Purity
- > 95%, as determined by mass spectrometry
- Formulation
- Lyophilized from 0.2 µm filtered solution of protein in DI-water.
- Endotoxin Level
- Less than 0.1 EU per µg cytokine as determined by the LAL method.
- Concentration
- Lyophilized, 10 µg per vial
- Storage & Handling
-
Lyophilized at -20°C to -80°C until expiration
Lyophilized at 2°C to 8°C for 2 weeks
Reconstituted at 2°C to 8°C for 2 weeks
Reconstituted at -20°C to -80°C for 3 months
Reconstitution: For maximum results, quick spin vial prior to opening. The protein can be aliquoted under aseptic conditions and stored at -20°C or colder. Stock solutions can be prepared at concentrations of 250 µg/mL up to 1 mg/mL in 0.22 µm filtered DI-water. Avoid repeated freeze/thaw cycles. - Activity
- Measured by its ability to recruit β-arrestin 2 in HEK293T cells transfected with human CXCR4. The ED50 for this effect was measured at 4 to 10 ng/mL.
- Application
-
FC, Live cell imaging
- Application Notes
-
Protein has been verified for bioactivity prior to conjugation
Antigen Details
- Structure
- CXCL12 is a 93 amino acids molecule with a predicted molecular weight of 9.9 kDa. PDB ID – 2J7Z
- Distribution
-
CXCL12 is expressed by intestinal epithelial cells, human brain micro vessel endothelial cells (HBMEC), bone marrow stromal and epithelial cells
- Function
- CXCL12 is chemotactic for both peripheral blood T cells and lamina propia T cells. CXCL12 is chemotactic for peripheral blood B cells from healthy and RA donors. CXCL12 and its receptor are involved in the mobilization of CD34+ progenitors to the peripheral blood and the homing of stem cells to their specific niches. CXCL12 induces proliferation in pancreatic intraepithelial neoplasia cells (PanIN). CXCL12 is a constitutive chemokine involved in lung, brain, joint, brain, and intestine inflammation
- Interaction
- SDF-1 binds to the CXCR4-expressed cells such as T lymphocytes, B cells monocytes, macrophages, tissue-committed stem/progenitor cells (TCSCs), mast cells, and vascular smooth muscle cells (VSMCs)
- Ligand/Receptor
- CXCL12 binds to CXCR4 receptor.
- Bioactivity
- Measured by its ability to recruit β-arrestin 2 in HEK293T cells transfected with human CXCR4. The ED50 for this effect was measured at 4 to 10 ng/mL
- Cell Type
- Leukocytes, Lymphocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, Platelets
- Biology Area
- Cell Biology, Immuno-Oncology, Immunology, Innate Immunity
- Molecular Family
- Cytokines/Chemokines, Innate Immune Signaling
- Antigen References
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- Shirozu M, et al. 1995. Genomics. 28:495-500.
- Dar A, et al. 2006. Exp Hemathol. 34:967-75.
- Müller A, et al. 2001. Nature. 410:50-6.
- Gelmini S, et al. 2008. J Endocrinol Invest. 31:809-19.
- Zlotnik A. 2008. J Pathol. 215:211-3.
- Gene ID
- P48061 View all products for this Gene ID
- UniProt
- View information about CXCL12 on UniProt.org
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