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Biotin anti-6-His Epitope Tag Antibody (Previously Covance catalog# BIOT-156L)

Pricing & Availability
Clone
6-His (See other available formats)
Regulatory Status
RUO
Other Names
polyhistidine-tag, hexa histidine-tag, 6xHis-tag, His6 tag, His-tag(TM)
Previously
Covance Catalog# BIOT-156L
Isotype
Mouse IgG1, κ
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Product Citations
publications
6-His_EpitopeTag_1_011918
Western blot analysis of 0.5 µl Posi-tag (Cat. No. 931301) using Biotin anti 6-His Mouse primary antibody (Clone 6-His, 1:2000 dilution, 0.5 µg/ml) and HRP Avidin secondary antibody (Cat. No. 405103, 1:1000 dilution).
  • 6-His_EpitopeTag_1_011918
    Western blot analysis of 0.5 µl Posi-tag (Cat. No. 931301) using Biotin anti 6-His Mouse primary antibody (Clone 6-His, 1:2000 dilution, 0.5 µg/ml) and HRP Avidin secondary antibody (Cat. No. 405103, 1:1000 dilution).
  • 6-His_EpitopeTag_2_011918
    Immunofluorescence of His tagged plasmid transfected A549 cells with (A) mouse IgG1, κ isotype control (Negative, Cat. No. 401402) or (B-D) Biotin-His Mouse primary antibody (Clone 6-His). Alexa Fluor® 594 (Red) Streptavidin (Cat. No. 405240) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/30, (B, D) is 1/500 and (C) is 1/800. Concentrations for (A-C) is 1 µg/ml, (D) is 0.5 µg/ml.
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906103 100 µL 419 CHF
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Description

His tags are commonly used as a method of tagging proteins for detection or purification. This antibody is raised again a polyhistidine tag (6-His or hexa His-tag).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal + 50% Glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

WB - Quality tested
ICC, IP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per mL (1:1000-1:2000 dilution). For immunocytochemistry, a concentration range of 0.5 - 1.0 μg/mL (1:1000-1:2000 dilution) is recommended. For immunoprecipitation, the suggested use of this reagent is 1:150 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The sequence of the protein flanking the poly His tag does not influence antibody binding. This antibody was subsequently purified via a protein-G column.

Application References

(PubMed link indicates BioLegend citation)
  1. Deinert K, et al. 2001. J Biol Chem. 276:6000. (WB)
  2. Kojima H, et al. 2001. J Biol Chem. 276:19238. (IF) PubMed
  3. Lapinski PE, et al. 2000. J Biol Chem. 275:6831. (WB)
  4. Bartsch D, et al. 1998. Cell. 95:211. (IF)
Product Citations
  1. Yang LF, et al. 2022. Anal Chem. 94:7278. PubMed
  2. Kojima H, et al. 2001. J Biol Chem. 276:19238-19243. PubMed
RRID
AB_2565057 (BioLegend Cat. No. 906103)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about 6-His on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 5    Revision Date: 09.19.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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