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Biotin anti-human C5a/C5a(desArg)/C5 Antibody

Pricing & Availability
Clone
G25/2 (See other available formats)
Regulatory Status
RUO
Other Names
C5AR1, C5A, C5AR
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
G25slash2_Biotin_020312
  • G25slash2_Biotin_020312
Cat # Size Price Quantity Check Availability Save
518305 50 µg 112 CHF
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518306 500 µg 347 CHF
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Description

C5, complement component 5, is a protein involved in the complement system. It is cleaved into C5a and C5b by the C3/C5 convertase. The smaller fragment C5a is an anaphylatoxin and  is released into the fluid surroundings. C5a is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. C5a also stimulates the locomotion of polymorphonuclear leukocytes (chemokinesis) and direct their migration toward sites of inflammation (chemotaxis). C5a contains 74 amino acids and is rapidly metabolised by a serum enzyme, carboxypeptidase B to a 73 amino acid form, C5a des-Arg. The major fragment C5b binds to the membrane initiating the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human C5a
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested
WB, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.25-1 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of C5a recombinant protein ranging from 15.6 to 1000 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The G25/2 antibody can neutralize the bioactivity of natural or recombinant C5a.

Neutralization: The G25/2 antibody can neutralize the bioactivity of natural or recombinant C5a/C5a(desArg)/C5. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human C5a/C5a(desArg)/C5 bioactivity in vivo and in vitro.

ELISA Detection: The biotinylated G25/2 antibody (Cat. No. 518306) is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified C17/5 antibody as the capture antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Oppermann M, et al. 1991. Kidney Int. 40:939947.
  2. Oppermann M, et al. 1992. Immunopharm. 24:119134.
  3. Elsner J, et al. 1994. Blood 83:3324.
RRID
AB_10896948 (BioLegend Cat. No. 518305)
AB_10896948 (BioLegend Cat. No. 518306)

Antigen Details

Biology Area
Cell Biology, Complement, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Lundwall AB, et al. 1985. J. Biol. Chem. 260:2108.
2. Fernandez HN, et al. 1978. J. Biol. Chem. 253:6955.
3. Tack BF, et al. 1979. Biochemistry 18:1490.

Gene ID
727 View all products for this Gene ID
UniProt
View information about C5a/C5adesArg/C5 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-human C5a/C5a(desArg)/C5

    G25slash2_Biotin_020312
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