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Biotin anti-human CXCL2 Antibody

Pricing & Availability
Clone
Poly5211 (See other available formats)
Regulatory Status
RUO
Other Names
Gro2, Macrophage inflammatory protein (MIP-2)
Isotype
Goat Polyclonal IgG
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Product Citations
publications
Poly5211_PURE_DKK-1_Direct-ELISA_Antibody_071023
Direct ELISA showing biotin anti-human CXCL2 (clone Poly5211) bound to plate-immobilized recombinant human CXCL2 protein (Cat. No. 582002). The wells were incubated with serially diluted biotin primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Avidin (Cat. No. 405103). TMB substrate (Cat. No. 421501) was used fordetection. Absorbance was measured at 450 nm.
  • Poly5211_PURE_DKK-1_Direct-ELISA_Antibody_071023
    Direct ELISA showing biotin anti-human CXCL2 (clone Poly5211) bound to plate-immobilized recombinant human CXCL2 protein (Cat. No. 582002). The wells were incubated with serially diluted biotin primary antibody at room temperature for 2 hours in 1% BSA-PBS, followed by incubation with HRP Avidin (Cat. No. 405103). TMB substrate (Cat. No. 421501) was used fordetection. Absorbance was measured at 450 nm.
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521103 50 µg 265 CHF
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Description

CXCL2 is an ELR CXC chemokine, and it is structurally and functionally related to GRO1 (CXCL1), GRO3 (CXCL3), and interleukin-8 (CXCL8). CXCL2 binds to the CXCR2 receptor; this receptor is shared with other ELR CXC chemokines (CXCL1, CXCL3, CXCL5, CXCL6, CXCL7, and CXCL8). CXCL2 is highly expressed in esophageal squamous cell carcinoma (ESCC), and it has been detected in serum of patients with this disease. CXCL1, CXCL2 and CXCL3 have all been shown to be highly expressed in patients with malignant melanoma. In addition, CXCL2 and CXCL1 are upregulated in the central nervous system in multiple sclerosis (MS) and in the animal model of experimental autoimmune encephalomyelitis (EAE). In EAE, it has been shown that transfer of encephalitogenic CD4+ Th17 cells induce CXCL1 and CXCL2 transcription in the spinal cords of naïve, syngeneic recipients. IL-17 is a potent inducer of ELR+ CXC chemokines in MS and EAE. It has been proven that CXCL1 and CXCL1 are upregulated in the preclinical and clinical stages of EAE.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Immunogen
Human Recombinant CXCL2 Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

Direct ELISA
Recommended Usage

Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.

Additional Product Notes

This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone Poly5211) could function as a detection antibody when paired with purified anti-human CXCL2 antibody (Cat. Nos. 311001 and 311002) (clone A16108E) as the capture antibody and using Recombinant Human CXCL2 (Groβ) protein (Cat. No. 582002) as the assay standard.

RRID
AB_3083210 (BioLegend Cat. No. 521103)

Antigen Details

Structure
Chemokine
Distribution

Macrophages, monocytes, fibroblasts, and epithelial cells

Function
CXCL2 chemoattracts and activates neutrophils, and it plays a key role in neutrophil mobilization from the bone marrow. CXCL2 is induced by TNFα, IL1α, IL-17, and LPS.
Interaction
Neutrophils, basophils, lymphocytes, monocytes, keratinocytes, hematopoitic cells, endothelial cells, bone marrow endolthelial cells, osteoblasts, epithelial cells, fibroblasts, melanoma cells, melanocytes, and neurons.
Ligand/Receptor
CXCR2
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Haskill S, et al. 1990. P Natl Acad Sci USA. 87:7732.
  2. De Plaen I, et al. 2006. Immunology. 118:153-63.
  3. Carlson T, et al. 2008. J Exp Med. 205:811-23.
  4. Eash KJ, et al. 2010. J Clin Invest. 120:2423-31.
  5. Keeley EC, et al. 2011. Exp Cell Res. 317:685-90.
  6. Onishi RM and Gaffen SL. 2011. Immunology. 129:311-21.
Gene ID
2920 View all products for this Gene ID
UniProt
View information about CXCL2 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All CXCL2 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CXCL2 Poly5211 Direct ELISA
Biotin anti-human CXCL2 Poly5211 Direct ELISA
Go To Top Version: 1    Revision Date: 06.16.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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