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Biotin anti-human IL-1α Antibody

Pricing & Availability
Clone
Poly5313 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-1α, Lymphocyte-activating factor (LAF), Endogenous pyrogen (EP), Leukocyte endogenous mediator (LEM), Mononuclear cell factor (MCF/MNCF)(il-1a,il1a)
Isotype
Goat Polyclonal Ig
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Product Citations
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Poly5313_Biotin_IL-1a_Antibody_032317
  • Poly5313_Biotin_IL-1a_Antibody_032317
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531304 50 µg 375 CHF
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Description

IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1α is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses. Recombinant human IL-1α is an 18.0 kD protein containing 159 amino acids.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant human IL-1α
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA. For ELISA detection applications, a concentration of 0.25 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-1α recombinant protein ranging from 200 to 1.56 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.

Application Notes

The biotinylated Poly5313 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified 343-3B3-14 (Cat. No. 500104) antibody as the capture antibody.

 

Note: For human IL-1a in cell culture supernatant, serum, plasma or other bodily fluids, BioLegend's LEGEND MAX™ Kits (Cat. No. 434907 & 434908) are specially developed and recommended.

RRID
AB_2686960 (BioLegend Cat. No. 531304)

Antigen Details

Structure
Cytokine
Distribution

Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglias, gliomas, keratinocyte

Function
Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
Interaction
B cells, T cells, monocytes, dendritic cells
Ligand/Receptor
Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Cell Type
Astrocytes, B cells, Dendritic cells, Endothelial cells, Fibroblasts, Langerhans cells, Macrophages, Monocytes, NK cells, T cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C et al. 1996. Blood 87:2095.

Gene ID
3552 View all products for this Gene ID
UniProt
View information about IL-1alpha on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 0    Revision Date: 04.24.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-human IL-1α

    Poly5313_Biotin_IL-1a_Antibody_032317
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