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Biotin anti-human MCP-1 (CCL2) Antibody

Pricing & Availability
Clone
Poly5360 (See other available formats)
Regulatory Status
RUO
Other Names
Monocyte chemoattractant protein-1 (MCP1), Monocyte chemoattractant and activating factor (MCAF), JE, Small inducible cytokine A2 (SCYA2), HC-11 (HC11), P6, Smooth muscle cell chemotactic factor (SMC-CF, SMCCF), CCL2, MCP-1, MCP1,CCL-2, MCP-1/CCL2
Isotype
Goat Ig
Ave. Rating
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Product Citations
publications
Poly5360_Biotin_MCP-1_ELISA_062918
  • Poly5360_Biotin_MCP-1_ELISA_062918
Cat # Size Price Quantity Check Availability Save
536004 50 µg 375 CHF
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Description

MCP-1, also known as CCL2, is a member of the CC chemokine family. It is widely expressed in endothelial cells, smooth muscle cells and monocytes in response to several atherogenic stimulants such as CD40 ligand, platelet derived growth factor (PDGF), interleukin-1β (IL-1β) and oxidized low density lipoprotein. In addition, MCP-1 has been implicated in monocytic infiltration of tissues during several inflammatory diseases, and has been implicated in macrophage-mediated tumor growth suppression in mice. MCP-1 has been shown to have direct effects on tumor cells in an autocrine and paracrine fashion in multiple cancers, including breast, lung, cervical, ovarian, and prostatic. In addition, MCP-1 plays a key role in the regulation of MMPs during transmigration.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Detection applications, a concentration range of 0.01 - 0.5 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 1000 to 15.6 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The biotinylated Poly5360 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified Poly5360 antibody (Cat. No. 536002) as the capture antibody and Recombinant Human MCP-1 (CCL2) (ELISA Std.) (Cat. No. 565001).

RRID
AB_2734497 (BioLegend Cat. No. 536004)

Antigen Details

Structure
CC chemokine, dimer, 13.8 kD (Mammalian)
Distribution

Monocytes, vascular endothelial cells, smooth muscle, glomerular mesangial cells

Function
Upregulated by IL-1, TNF-α, PDGF, TGF-β
Interaction
Monocytes, basophils, T cells, NK cells, immature dendritic cells
Ligand/Receptor
CCR2, CCR5, CCR10, D6, possibly L-CCR
Cell Type
Endothelial cells, Monocytes
Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald KA, et al. 2001. The Cytokine FactsBook; Academic Press, San Diego.
2. Bischoff SC, et al. 1992. J. Exp. Med. 175:1271.
3. Charo IF, et al. 1994. Proc. Natl. Acad. Sci. USA. 91:2752.
4. Jiang Y, et al. 1992. J. Immunol. 148:2423.

Gene ID
6347 View all products for this Gene ID
UniProt
View information about MCP-1 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 06.28.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human MCP-1 (CCL2)

    Poly5360_PURE_MCP-1_ELISA_062918

  • Biotin anti-human MCP-1 (CCL2)

    Poly5360_Biotin_MCP-1_ELISA_062918
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