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Biotin anti-human NGAL (Lipocalin-2) Antibody

Pricing & Availability
Clone
M049D6 (See other available formats)
Regulatory Status
RUO
Other Names
Lipocalin 2, Oncogene 24p3, Neutrophil Gelatinase-Associated Lipocalin, Siderocalin LCN2, 25 kD Alpha-2-Microglobulin-Related Subunit Of MMP-9, p25, MSFI, Migration-Stimulating Factor Inhibitor, HNL
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
M049D6_Biotin_NGAL_Lipocalin-2_Antibody_040317
  • M049D6_Biotin_NGAL_Lipocalin-2_Antibody_040317
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503304 500 µg 469 CHF
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Description

NGAL (lipocalin-2) is a lipocalin family protein that plays important roles in innate immunity, inflammation, and atherosclerosis. It was originally identified as a 25 kD protein covalently bound to gelatinase from human neutrophils. NGAL is a critical component of innate immunity because of its ability to bind iron-siderophore complexes and limit bacterial growth. It is also produced by injured epithelia and is one of the most promising markers for renal epithelial injury and cardiovascular disease (CVD).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human NGAL
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA. For ELISA detection applications, a concentration of 0.15 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of NGAL recombinant protein ranging from 2500 to 39.1 pg/mL is recommended for each ELISA plate.

It is recommended that the reagent be titrated for optimal performance.

Application Notes

ELISA Detection: The biotinylated M049D6 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified M0415B7 (Cat. No. 661902) antibody as the capture antibody.

 

Note: For testing human NGAL in cell culture supernatant, serum, plasma, urine, saliva, and other biological fluids, BioLegend's LEGEND MAX™ Kits (Cat. No. 443407 & 443408) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Iqbal N, et al. 2013. Expert Opin. Med Diagn. 7:209.
  2. Singer E, et al. 2013. Acta. Physiol. (Oxf) 207:663.
  3. Palazzuoli A, et al. 2012. Clin. Chim. Acta. 413:1832.
  4. Flo TH, et al. 2004. Nature 432:917
  5. Goetz DH, et al. 2002. Mol. Cell. 10:1033.
  6. Leung L, et al. 2012. PLoS One 7:e46677
  7. Kjeldsen L, et al. 1993. J. Biol. Chem. 268:10425.
RRID
AB_2650710 (BioLegend Cat. No. 503304)

Antigen Details

Structure
193 amino acid monomer with a predicted molecular weight of approximately 22 kD.
Distribution

Secreted by epithelial cells, macrophages, neutrophils. Increased levels have been observed in plasma, serum, and urine in various conditions, such as metastatic breast and colorectal cancer, acute kidney injury, pancreatitis and preeclampsia.

Function
Apoptosis, innate immunity, renal development.
Interaction
Disulfide-linked with MMP9
Ligand/Receptor
SLC22A17 (24p3R)
Cell Type
Epithelial cells, Macrophages, Neutrophils
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Gene ID
3934 View all products for this Gene ID
UniProt
View information about NGAL on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 0    Revision Date: 04.04.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-human NGAL (Lipocalin-2)

    M049D6_Biotin_NGAL_Lipocalin-2_Antibody_040317
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