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Biotin anti-human PlGF Antibody

Pricing & Availability
Clone
24G3 (See other available formats)
Regulatory Status
RUO
Other Names
PlGF, PLGF, Placental Growth Factor
Isotype
Mouse IgG2a
Ave. Rating
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Product Citations
publications
24G3_Biotin_PIGF_Antibody_121418
  • 24G3_Biotin_PIGF_Antibody_121418
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534904 100 µg 265 CHF
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Description

Placental growth factor (PlGF) is a member of the PDGF/VEGF family of growth factors. Four alternatively-spliced isoforms have been identified: PlGF-1, PlGF-2, PlGF-3, and PlGF-4. All isoforms are expressed in placental tissues, with PlGF-2 being expressed specifically during early placental development. PlGF-1 and PlGF-3 are considered diffusible isoforms, while PlGF-2 and PlGF-4 each contain a unique heparin binding domain that may increase associations with cell membranes. In addition to placental tissues, PlGF is expressed in the microvasculature, bone marrow, umbilical vein endothelia, uterine keratinocytes, and NK cells. PlGF binds to both VEGF-R1 and neuropilin, but not VEGF-R2 and induces monocyte activation, migration, inflammatory cytokine production. PlGF plays a key role in maternal vascular function during pregnancy, with circulating levels reaching a peak mid-gestation. It can be used as a marker for pregnancy-associated hypertensive disorders such as pre-eclampsia.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA-D - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Detection applications, a concentration range of 0.1 - 4.0 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The biotin 24G3 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with pure 13C8 antibody (Cat. No. 534802) as the capture antibody.

RRID
AB_2894609 (BioLegend Cat. No. 534904)

Antigen Details

Structure
Homodimer
Distribution

Vascular endothelial cells, smooth muscle cells, keratinocytes, hematopoitic cells, retinal pigment epithelial cells, tumor cells, and cerebellar stroma cells.

Function
PlGF is an angiogenic factor and induces proliferation and migration of endothelial cells. It stimulates endothelial cells and synergistically amplifies the action of VEGF. Soluble VEGFR1 (sFLT1) can regulate the accessibility of PlGF.
Interaction
Endothelial cells, Macrophages, and trophoblats.
Ligand/Receptor
VEGFR1 (FLT-1), and neuropilin 1 (Nrp1)
Cell Type
Endothelial cells, Epithelial cells, Hematopoietic stem and progenitors
Antigen References
  1. Maglione D, et al. 1991. Proc Natl Acad Sci. USA 88:9267.
  2. Carmeliet P, et al. 2001. Nat Med. 7:575.
  3. Casalou C, et al. 2007. Leukemia. 21:1590.
  4. Marcellini M, et al. 2006. Am J Pathol. 169:643.
  5. Yao J, et al. 2011. Proc Natl Acad Sci USA. 108:11590.
  6. Cai J, et al. 2011. PLoS One. 6:e18076.
  7. Sibiude J, et al. 2012. PLoS One. 7:e50208.
  8. Snuderl M, et al. 2013. Cell. 152:1065.
Gene ID
5228 View all products for this Gene ID
UniProt
View information about PlGF on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 12.14.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-human PlGF

    24G3_Biotin_PIGF_Antibody_121418
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