- Clone
- N382/14 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Mitochondrial Fission Factor, C2orf33, Chromosome 2 Open Reading Frame 33, EMPF2, GL004
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
857505 | 25 µg | 106 CHF | ||||
857506 | 100 µg | 188 CHF |
Mitochondrial fission factor (MFF) is a mitochondrion outer membrane protein that is part of the mitochondrial fission complex where it acts a receptor for the dynamin-related protein 1 (Drp1). The MFF-Drp1 complex is responsible for the promotion of mitochondrial fission. MFF has also been shown to regulate peroxisome morphology.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Fusion protein including amino acids 1-173 and 272-322 of human MFF
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ICC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2820141 (BioLegend Cat. No. 857505)
AB_2820141 (BioLegend Cat. No. 857506)
Antigen Details
- Structure
- MFF is a 342 amino acid protein with a molecular mass of ~38 kD.
- Distribution
-
Tissue Distribution: Highly expressed in heart, kidney, liver, brain, muscle, and stomach.
Cellular Distribution: Mitochondria, peroxisome, nucleus, and cytosol. - Function
- MFF plays a role in mitochondrial and peroxisomal fission by facilitating the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface.
- Interaction
- MFF interacts with the dynamin-related protein 1 (Drp1). MFF has also been shown to interact with DDX28 and ILF3.
- Biology Area
- Cell Biology, Mitochondrial Function, Neuroinflammation, Neuroscience
- Molecular Family
- Organelle Markers
- Antigen References
-
- Moon Yong Cha, et al. 2018. PLoS Curr. 26;10.
- Kornfeld OS, et al. 2018. Sci Rep. 19;8(1):14034.
- Otera H, et al. 2010. J Cell Biol. 13;191(6):1141-58.
- Liu R, et al. 2015. Mol Biol Cell. 1;26(24):4466-77.
- Gene ID
- 56947 View all products for this Gene ID
- UniProt
- View information about MFF on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Other Formats
View All MFF Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-MFF | N382/14 | WB,IHC-P |
Biotin anti-MFF | N382/14 | ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-MFF
Western blot of purified anti-MFF antibody (clone N382/14). ... IHC staining of purified anti-MFF antibody (clone N382/14) o... IHC staining of purified anti-MFF antibody (clone N382/14) o... IHC staining of purified anti-MFF antibody (clone N382/14) o... IHC staining of purified anti-MFF antibody (clone N382/14) o... -
Biotin anti-MFF
ICC staining of Biotin anti-MFF antibody (clone N328/14) on ... ICC staining of Biotin anti-MFF antibody (clone N328/14) on ...
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