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Biotin anti-mouse CD185 (CXCR5) Antibody

Pricing & Availability
Clone
L138D7 (See other available formats)
Regulatory Status
RUO
Other Names
BLR1, GPCR6, MDR-15
Isotype
Rat IgG2b, κ
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Product Citations
publications
L138D7_Biotin_CXCR5_Antibody_FC_1_052313
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC and biotinylated CXCR5 (clone L138D7, top) or rat IgG2b, κ isotype control (bottom), followed by Sav-PE.
  • L138D7_Biotin_CXCR5_Antibody_FC_1_052313
    C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC and biotinylated CXCR5 (clone L138D7, top) or rat IgG2b, κ isotype control (bottom), followed by Sav-PE.
  • L138D7_Biotin_CXCR5_Antibody_FC_2_052313
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Cat # Size Price Quantity Check Availability Save
145509 25 µg 112 CHF
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145510 100 µg 293 CHF
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Description

CD185 is also known as CXCR5. It is the receptor for chemokine CXCL13/BLC, which is chemotactic for B cells.  CXCR5 is expressed on B cells and a subset of T cells in the spleen, neuronal tissue, lymph nodes, and bone marrow.  It is important for migration of B cells into the B cell follicles of the spleen and Peyer’s patches.  Follicular helper T cells (Tfh) also express CXCR5 and the ability of these cells to migrate to the lymph node is modulated by the balanced expression of CCR7 and CXCR5.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
mCXCR5-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone L138D7 staining works optimally at room temperature or 4°C. Unlike other chemokine receptor antibodies, avoid using L138D7 at 37°C.

Product Citations
  1. Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed
  2. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
  3. Du J, et al. 2022. Sci Immunol. 7:eabo5407. PubMed
  4. Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed
  5. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  6. Chen JS, et al. 2022. Sci Immunol. 7:eabl5652. PubMed
  7. Kim Y, et al. 2015. PLoS One. 10:120294. PubMed
  8. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  9. Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
  10. Kato Y, et al. 2020. Immunity. 53(3):548-563. PubMed
  11. Yang K, et al. 2018. J Virol. 92:e00428. PubMed
  12. Biram A, et al. 2020. Bio Protoc. e3562:10. PubMed
  13. Zeiträg J, et al. 2021. Eur J Immunol. 51:408. PubMed
  14. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  15. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  16. Reimer D, et al. 2020. Cell Reports. 32(6):108030. PubMed
  17. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  18. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  19. Alterauge D, et al. 2020. Cell Rep. 33:108232. PubMed
  20. Katsuyama T, et al. 2021. Cell Reports. 36(1):109339. PubMed
  21. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
RRID
AB_2562125 (BioLegend Cat. No. 145509)
AB_2562125 (BioLegend Cat. No. 145510)

Antigen Details

Structure
42 kD, seven transmembrane protein
Distribution

B cells and a subset of T cells in mouse spleen, Tfh cells, also expressed in lymph node, bone marrow, and brain

Function
B cell migration into follicles, possible regulatory role in B cell differentiation and Burkitt's lymphoma
Ligand/Receptor
BLC/CXCL13
Cell Type
B cells, T cells, Tfh
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Kaiser E, et al. 1993. Eur. J. Immunol. 23:2532.
2. Forster R, et al. 1994. Cell. Mol. Biol. 40:381.
3. Forster R, et al. 1994. Blood 84:830.
4. Forster R, et al. 1996. Cell 87:1037.
5. Wolf I, et al. 1998. J. Biol. Chem. 273:28831.

Gene ID
12145 View all products for this Gene ID
UniProt
View information about CD185 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 05.23.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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