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Biotin anti-mouse CD355 (CRTAM) Antibody

Pricing & Availability
Clone
11-5/CRTAM (See other available formats)
Regulatory Status
RUO
Other Names
Class I-restricted T cell-associated molecule
Isotype
Rat IgG2a, κ
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Product Citations
publications
11-5-CRTAM_Biotin_CD355_Antibody_1_101917
C57BL/6 mouse splenocytes were stimulated (overnight) with immobilized TCR-β (clone H57-597). Cell were stained with CD8 FITC and biotinylated CD355 (clone 11-5/CRTAM) (top) or rat IgG2a, κ isotype control (bottom), followed by Streptavidin PE.
  • 11-5-CRTAM_Biotin_CD355_Antibody_1_101917
    C57BL/6 mouse splenocytes were stimulated (overnight) with immobilized TCR-β (clone H57-597). Cell were stained with CD8 FITC and biotinylated CD355 (clone 11-5/CRTAM) (top) or rat IgG2a, κ isotype control (bottom), followed by Streptavidin PE.
  • 11-5-CRTAM_Biotin_CD355_Antibody_2_101917
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142012 100 µg 265 CHF
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Description

Class-I MHC-restricted T-cell associated molecule (CRTAM), designated as CD355, is an Ig domain like type I transmembrane receptor, which belongs to the nectin family. In the immune system, CRTAM is strictly expressed on activated NK cells and activated CD8-positive T cells. By binding its ligand Nectin-like molecule 2 (Necl2), CRTAM plays an important role in mediating cell adhesion and migration.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CRTAM extracellular region-human IgG Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats) include:  Western blotting1.

Application References

(PubMed link indicates BioLegend citation)
  1. Arase N, et al. 2005. Int. Immunol. 17:1227. (WB)
Product Citations
  1. Potempa M, et al. 2022. J Immunol. 208:1742. PubMed
RRID
AB_2721503 (BioLegend Cat. No. 142012)

Antigen Details

Structure
75 kD homodimer, nectin family, Ig superfamily
Distribution

Activated NK cells and activated CD8-positive T cells

Function
Cell adhesion, migration
Ligand/Receptor
Nectin-like molecule 2
Cell Type
NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Takeuchi A, et al. 2009. J. Immunol. 183:4220.
2. Yeh JH, et al. 2008. Cell 132:846.
3. Arase N. 2005. Int. Immunol. 17:1227.
4. Boles KS, et al. 2005. Blood 106:779.
5. Galibert L, et al. 2005 J. Biol. Chem. 280:21955.
6. Kennedy J, et al. 2000 J. Leukoc. Biol. 67:725.

Gene ID
54698 View all products for this Gene ID
UniProt
View information about CD355 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 0    Revision Date: 10.20.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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