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Biotin anti-mouse/human PNAd Antibody

Pricing & Availability
Clone
MECA-79 (See other available formats)
Regulatory Status
RUO
Other Names
PNAd, Peripheral Node Addressin, MECA-79
Isotype
Rat IgM, κ
Ave. Rating
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Product Citations
publications
MECA-79_Biotin_PNAd_Antibody_1_032822
C57BL/6 mouse frozen lymph node section was fixed with 4% paraformaldehyde (PFA) for ten minutes and blocked with 5% FBS plus 5% rat/mouse serum for 30 minutes at room temperature. Then the section was stained with 5 µg/mL of biotin anti-mouse/human PNAd antibody (clone MECA-79) overnight at 4°C. On the next day, the tissue was washed twice with PBS and stained with 2 µg/mL of Alexa Fluor® 647 Streptavidin (red) and Alexa Fluor® 594 anti-mouse CD31 antibody (clone 390) for a half hour at room temperature. The image was captured with a 10X objective.
  • MECA-79_Biotin_PNAd_Antibody_1_032822
    C57BL/6 mouse frozen lymph node section was fixed with 4% paraformaldehyde (PFA) for ten minutes and blocked with 5% FBS plus 5% rat/mouse serum for 30 minutes at room temperature. Then the section was stained with 5 µg/mL of biotin anti-mouse/human PNAd antibody (clone MECA-79) overnight at 4°C. On the next day, the tissue was washed twice with PBS and stained with 2 µg/mL of Alexa Fluor® 647 Streptavidin (red) and Alexa Fluor® 594 anti-mouse CD31 antibody (clone 390) for a half hour at room temperature. The image was captured with a 10X objective.
  • MECA-79_Biotin_PNAd_Antibody_2_032822
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH7.4) at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of biotin anti-mouse/human PNAd antibo
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120803 50 µg 83 CHF
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120804 500 µg 282 CHF
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Description

MECA-79 recognizes a carbohydrate epitope shared with a group of sulfation decorated sialomucins, including sulfated ligands for CD62L (CD34, GlyCAM-1, Sgp200, and a subset of MAdCAM-1). This set antigens has been referred to as peripheral node addressin (PNAd) with the molecular mass 50-250 kD. It has been identified that GlcNAc-6-SO4 sulfation contributes to MECA-79 binding and the core 1 β1,3-N-acetylglucosaminyltransferase is required for the generation of the MECA-79 epitope. MECA-79 is expressed on high endothelial venules (HEV) of lymphoid tissues, chronic inflamed tissues and rheumatoid synovia. The interaction of PNAd with CD62L receptor is involved in tethering and rolling of lymphocytes along HEV in lymphoid tissues. MECA-79 antibody reacts with mouse, human and many other species PNAd and blocks L-selectin-dependent lymphocyte adhesion in vitro and in vivo.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse lymph node stromal cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

IHC-F - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per mL. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 5.0 - 10 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking1-4 of cell adhesion, immunohistochemical staining1,2,6 of acetone-fixed frozen sections and 4% paraformaldehyde-fixed paraffin-embedded sections, flow cytometry10,11, immunoprecipitation3,5, and Western blotting6,11.

Application References

(PubMed link indicates BioLegend citation)
  1. Streeter PR, et al. 1988. J. Cell Biol. 107:1853. (IHC, Block)
  2. Michie SA, et al. 1993. Amer. J. Path. 143:1688. (IHC, Block)
  3. Berg EL, et al. 1991. J. Cell Biol. 114:343. (IP, Block)
  4. Berg EL, et al. 1993. Nature 366:695. (Block)
  5. Hemmerich S, et al. 1994. J. Exp. Med. 180:2219. (IP)
  6. Samulowitz U, et al. 2002. Am. J. Path. 160:1669. (IHC, WB)
  7. Burke SD, et al. 2007. Diabetes 56:2919. PubMed
  8. Hirakawa J, et al. 2010. J. Biol. Chem. 285:40864. PubMed
  9. Thomas SN, et al. 2012. J. Immunol. 189:2181. PubMed.
  10. Izawa D, et al. 1999. Int. Immunol. 11(12):1989-98. (FC, ICC)
  11. Sinha RK, et al. 2006. Immunology. 119(4):461-9. (FC, IHC-F, WB)
Product Citations
  1. Lütge M, et al. 2023. Nat Immunol. . PubMed
  2. Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
  3. Folgosa L, et al. 2013. J Immunol. 191:5951. PubMed
  4. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  5. van Hooren L, et al. 2021. Nature Communications. 12(1):4127. PubMed
  6. Onder L et al. 2017. Immunity. 47(1):80-92 . PubMed
  7. Xiong H, et al. 2021. Theranostics. 11:1594. PubMed
  8. Bombardieri M, et al. 2012. J Immunol. 189:3767. PubMed
  9. He W, et al. 2020. Frontiers in Oncology. 1.097222222. PubMed
  10. Ligon MM, et al. 2020. Mucosal Immunol. 1.172222222. PubMed
  11. Rodriguez AB, et al. 2022. Front Immunol. 13:1009306. PubMed
  12. Tsuboi K, et al. 2013. J Immunol. 191:448. PubMed
  13. Yan X, et al. 2015. PLoS One. 10: 0145218. PubMed
  14. Huppé CA, et al. 2018. Mucosal Immunol. 0.536111111. PubMed
RRID
AB_493556 (BioLegend Cat. No. 120803)
AB_493556 (BioLegend Cat. No. 120804)

Antigen Details

Structure
Sulphated CD34, GlyCAM-1 and MAdCAM-1, Sgp200, sialomucin
Distribution

High endothelial venules (HEV) in lymphoid tissues, inflamed tissues and rheumatoid synovia

Function
Leukocyte homing, rolling
Ligand/Receptor
CD62L
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules
Antigen References

1. Streeter PR, et al. 1988. J. Cell Biol. 107:1853.
2. Berg EL, et al. 1993. Nature 366:695.
3. Hemmerich S, et al. 1994. J. Exp. Med. 180:2219.
4. Mitoma J, et al. 2003. J. Biol. Chem. 278:9953.
5. Bruehl RE, et al. 2000. J. Biol. Chem. 275:32642.
6. Bistrup A, et al. 2004. Am. J. Pathol. 164:1635

Gene ID
123803 View all products for this Gene ID 18203 View all products for this Gene ID
UniProt
View information about PNAd on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 3    Revision Date: 06.15.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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