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Biotin anti-mouse IgEa Antibody

Pricing & Availability
Clone
UH297 (See other available formats)
Regulatory Status
RUO
Other Names
1. Usui, M.
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
408804 500 µg 253 CHF
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Description

The UH297 antibody reacts specifically with mouse soluble immunoglobulin E of Igh-Ca haplotype (e.g., A, AKR, BALB/c, C3H/He, CBA). It does not react with IgE Igh-cb haplotype (e.g., C57BL/6, C57BL/10, SJL) and other Ig isotypes. UH297 mAb can be used for immunofluorescent staining of cell surface IgEa and ELISA applications.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse IgE anti-DNP mAb from DNPε 117 hybridoma
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA - Quality tested
FC, WB - Reported in the literature, not verified in house
Recommended Usage
Each lot of this antibody is quality control tested by ELISA. For ELISA detection, the suggested use of this reagent is 1-2 µg per ml. It's recommended that the reagent be titrated for optimal performance for each application.
Application References

(PubMed link indicates BioLegend citation)
  1. Usui M, et al. 1993. Immunogenetics. 37:301-304
  2. Yamada M, et al. 1994. Immunogenetics. 40:296-299
Product Citations
  1. Kim JY, et al. 2023. J Immunol Res. 2023:9024595. PubMed
  2. Cárdenas–Torres FI, et al. 2019. Medicina (Kaunas). 55:3. PubMed
  3. Choi JH, et al. 2021. Biomed Res Int. 2021:2522305. PubMed
RRID
AB_1937222 (BioLegend Cat. No. 408804)

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-mouse IgEa

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