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Biotin anti-mouse IL-22 Antibody

Pricing & Availability
Clone
Poly5164 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-22, IL-10-related T cell-derived inducible factor, IL-TIF
Isotype
Goat Polyclonal IgG
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Product Citations
publications
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516407 50 µg 375 CHF
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Description

IL-22 is a cytokine structurally related to IL-10.  Mouse IL-22 consists of 179 amino acids and has a predicted molecular weight of 20 kD.  It is secreted primarily by Th17, Th1, Th2, lymphoid tissue inducer cells (LTi), and subsets of natural killer cells.  It has been reported that aryl hydrocarbon receptor (AhR) expression is essential for the production of IL-22 by TCRγδ T cells.  AhR activation increases Th17 polarization and induces IL-22 production.  IL-22 functions by engaging the heterodimeric IL-22 receptor (IL-22R) complex, consisting of two receptor subunits, IL-22R1 and IL-10Rβ.  IL-22 acts on nonhematopoietic tissue cells, such as epithelial cells of the digestive and respiratory systems and keratinocytes of the skin.  IL-22 is involved in inflammatory processes such as dermal inflammation, psoriasis, inflammatory bowel disease, hepatitis, and Crohn’s disease.  Moreover, it plays a critical role in mucosal immunity and the wound healing process.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Mouse IL-22, amino acids Leu34-Val179 (Accession# NM_016971), was expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.2-1 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance. To obtain a linear standard curve, serial dilutions of IL-22 recombinant protein ranging from 250 to 3.9 pg/ml are recommended.

Application Notes

ELISA Capture: The purified Poly5164 antibody is useful as a capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5164 antibody (Cat. No. 516407) as the detecting antibody and recombinant mouse IL-22 (Cat. No. 576209) as the standard.

In sandwich ELISA, Poly5164 has approximately 17% and 2% cross-reactivity with recombinant rat and human IL-22, respectively.

Flow Cytometry: The fluorochrome-labeled Poly5164 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify IL-22-producing cells within mixed cell populations.

Note: For testing mouse IL-22 in serum, plasma or supernatant, LEGEND MAX™ Mouse IL-22 ELISA Kit (Cat. No. 436307 & 436308) is specially developed and recommended.

Note: The use of Iscove's Modified Dulbecco's Medium (IMDM) will result in better in vitro Th17 polarization.1

Application References

(PubMed link indicates BioLegend citation)
  1. Veldhoen M, et al. 2009. J. Exp. Med. 206:43.
Product Citations
  1. Rao D, et al. 2023. Eur J Immunol. 53:e2250258. PubMed
RRID
AB_2561414 (BioLegend Cat. No. 516407)

Antigen Details

Structure
A cytokine structurally related to IL-10 with a predicted molecular weight of 20 kD.
Distribution

Th17, Th1, Th2, lymphoid tissue inducer cells (LTi), and subsets of natural killer cells.

Function
Acts on nonhematopoietic tissue cells, such as epithelial cells and keratinocytes, involved in inflammatory processes, mucosal immunity, and wound healing.
Cell Type
NK cells, Th1, Th17, Th2
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Martin B, et al. 2009. Immunity 31:321.
2. Liang SC, et al. 2006. J. Exp. Med. 203:2271.
3. Veldhoen M, et al. 2008. J. Exp. Med. 206:43.
4. Zheng Y, et al. 2007. Nature 445:648.
5. Dumoutier L, et al. 2000. J. Immunol. 164:1814.
6. Wolk K, et al. 2007. J. Immunol. 178:5973.

Gene ID
50929 View all products for this Gene ID
UniProt
View information about IL-22 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 5    Revision Date: 02.09.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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