Biotin anti-mouse Lypd8 Antibody anti-Lypd8

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Clone: SA025A4 (See other available formats)
Regulatory Status: RUO
Other Names: Ly6/PLAUR domain containing 8
Isotype: Mouse IgG1, κ
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Product Citations: publications

SA025A4_Biotin_Lypd8_Antibody_103116 — Mouse Lypd8-transfected cells were stained with biotinylated Lypd8 (clone SA025A4, filled histogram) or biotinylated mouse IgG1, κ isotype control (open histogram), followed by Sav-PE.

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152704

100 µg

305 CHF

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Description

Ly6/PLAUR domain containing 8 (Lypd8) is a 100-120 kD GPI-anchored glycoprotein and a member of the Ly6/PLAUR family. Lypd8 is highly expressed on epithelial cells from intestinal lumen, where it prevents bacterial invasion by inhibiting bacterial motility by binding to flagella.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Mouse Lypd8-transfected cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone does not recognize Lypd8 from BALB/c mice.
RRID: AB_2632692 (BioLegend Cat. No. 152704)

Antigen Details

Structure: GPI-anchored, glycoprotein, 100-120 kD.
Distribution: Epitelials cells in cecum and large intestine.
Function: Inhibits bacterial motility by binding to flagella.
Cell Type: Epithelial cells
Biology Area: Immunology, Innate Immunity
Antigen References: 1. Okumura R, et al. 2016. Nature 532:117.
2. Loughner CL, et al. 2016. Hum. Genomics 10:10.
Gene ID: 70163 View all products for this Gene ID
UniProt: View information about Lypd8 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

How many biotin molecules are per antibody structure?: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All Lypd8 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse Lypd8	SA025A4	FC,IHC-F,IHC-P
Biotin anti-mouse Lypd8	SA025A4	FC
Alexa Fluor® 594 anti-mouse Lypd8	SA025A4	IHC-F
Alexa Fluor® 647 anti-mouse Lypd8	SA025A4	IHC-F
PE anti-mouse Lypd8	SA025A4	FC

Certificate of Analysis

Go To Top Version: 2 Revision Date: 05.26.2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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