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Biotin anti-mouse/rat β-Amyloid, 1-16 Antibody (Previously Covance catalog# SIG-39156)

Pricing & Availability
Clone
M3.2 (See other available formats)
Regulatory Status
RUO
Other Names
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously
Covance Catalog# SIG-39156
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
Cat # Size Price Quantity Check Availability Save
805704 1 mL 2270 CHF
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805703 200 µL 652 CHF
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Description

Alzheimer's disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APP) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The epitope has been determined to be within residues 10-15 of murine Aβ.
Formulation
Phosphate-buffered solution; no preservatives or carrier proteins.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
ICC - Reported in the literature, not verified in house
ELISA
Recommended Usage

Each lot of this antibody is quality control tested by western blotting.

The optimal working dilution should be determined for each specific assay condition.
• WB: 1:50,000-1:200,000
• IF: 1:1,000-1:2,000

Application Notes

Clone M3.2 recognizes a rodent sequence of Aß, ß-CTF, sAPP-a, and full length APP. Clone 3.2 has negligible cross reactivity with human isoforms of APP and beta amyloid.

Additional Product Notes

Expected MW: APP & sAPPalpha = 100 kD, Beta-CTF = 10 kD, Beta Amyloid = 4 kD

Application References

(PubMed link indicates BioLegend citation)
  1. Morales-Corraliza J, et al.  2009. PLoS One 4(9):e7134. (IP, WB, ELISA Detection)
  2. Teich AF, et al. 2013. PLoS One 8(2):e55647, 2013. (ELISA Capture, WB)
  3. Lee Y, et al. 2017. Neuropharmacology. 117:387-400. (ICC)
  4. Lundgren JL, et al. 2015. J Neurochemistry. 135:606-15. (ICC)
Product Citations
  1. Currais A, et al. 2012. Aging Cell. 11:1017-1026. PubMed
RRID
AB_2564983 (BioLegend Cat. No. 805704)
AB_2564983 (BioLegend Cat. No. 805703)

Antigen Details

Structure
Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
Distribution

Tissue sources: Primarily nervous system, but also adipose tissue, intestine, muscle.

Distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function
The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Antigen References
  1. Kumar A, et al. 2015. Pharmacol. Rep. 67(2):195.
  2. Sadigh-Eteghad S, et al. 2015. Med. Princ. Pract. 24(1):1.
  3. Hampel H, et al. 2015. Expert Rev. Neurother. 15(1):83.
  4. Puig KL, Combs CK. 2013. Exp. Gerontol. 48(7): 608.
  5. Selkoe DJ, Hardy J. 2016. EMBO Mol. Med. 8(6):595.
  6. Walsh DM, Selkoe DJ. 2007. J. Neurochem. 101(5):1172.
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid 1-16 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 5    Revision Date: 06.11.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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