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Biotin anti-SNAP-25 Antibody

Pricing & Availability
Clone
SMI 81 (See other available formats)
Regulatory Status
RUO
Other Names
Synaptosomal-associated protein 25, SUP, Synaptosomal-associated 25 kD Protein
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SMI-81_SNAP25_Biotin_Antibody_1_102417
Western blot of Biotin anti-SNAP-25 antibody (clone SMI 81). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1.0 µg/mL of primary antibody overnight at 4°C, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system.
  • SMI-81_SNAP25_Biotin_Antibody_1_102417
    Western blot of Biotin anti-SNAP-25 antibody (clone SMI 81). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 1.0 µg/mL of primary antibody overnight at 4°C, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system.
  • SMI-81_SNAP25_Biotin_Antibody_2_102417
    IHC staining of Biotin anti-SNAP-25 antibody (clone SMI 81) on formalin-fixed paraffin-embedded normal rat brain tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 3: Acidic, 1X (Cat. No. 927701), the tissue was incubated with the primary antibody at 5 µg/ml for 1 hour at room temperature. For detection, the HRP labeling reagent and DAB from BioLegend Ultra Streptavidin (USA) HRP Detection Kit was used (Multi-Species, component No. 5, DAB; Cat. No. 929901). The tissue was counterstained with hematoxylin and bluing solution. Image was taken using 20X objective.
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836307 25 µg 118 CHF
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836308 100 µg 300 CHF
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Description

Synaptosomal-associated protein 25 (SNAP-25) is a component of the trans-SNARE (t-SNARE) complex. SNAP-25 has been reported to account for the specificity of membrane fusion, and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together. SNAP-25 has been shown to inhibits both ATP-dependent and independent Ca2+-triggered release of glutamate from central nervous system (CNS) synaptosomal membranes. This indicates that SNAP-25 has a role not only in the formation of the synaptic vesicle-target v- and t-SNARE complex, but also in the final neurotransmitter release.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length, unaltered SNAP-25
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 to 5.0 µg/ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Postupna NO, et al. 2014. Lab Invest. 94:1161. (FC)
  2. Hirano AA, et al. 2011. J. Comp. Neurol. 519:972. (WB, IHC, EM) PubMed
  3. Flores-Otero J, et al. 2007. J. Neurosci. 27:14023. (IF) PubMed
  4. Rozas JL, et al. 2012. Neuron. 74:151. (IF) PubMed
  5. Brinkmalm A, et al. 2014. Mol. Neurodegener. 9:53. (IP) PubMed
  6. Gu Y, et al. 2016. Proc. Natl. Acad. Sci. U. S. A. E922-31. (WB) PubMed
  7. Kabachinski G, et al. 2016. Mol. Biol. Cell. 654-68.(IF) PubMed
  8. Kunii M, et al. 2016. J. Cell Biol. 215:121-138. (IP) PubMed
RRID
AB_2721508 (BioLegend Cat. No. 836307)
AB_2721508 (BioLegend Cat. No. 836308)

Antigen Details

Structure
SNAP-25 is a 206 amino acid protein with a molecular mass of 23 kD.
Distribution

Tissue distribution: Mainly in the central nervous system, in neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum
Cellular distribution: Cytosol, golgi apparatus, and cell membrane

Function
SNAP-25 associates with proteins involved in vesicle docking and membrane fusion. It regulates plasma membrane recycling through its interaction with centromere protein F (CENPF).
Interaction
In adult tissue, SMI 81 visualizes synapse-rich areas of brain and retina. In developing brain and cell cultures, SMI 81 reacts with neuronal cell bodies and axons. It is useful as an early marker for developing neurons in cell culture.
Cell Type
Neurons
Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
Molecular Family
Presynaptic proteins
Antigen References

1. Snyder DA, et al. 2006. Cell Biochem. Biophys. 45(1):111-23.
2. Nagy G, et al. 2004. Neuron. 41(3):417–29.

Gene ID
6616 View all products for this Gene ID
UniProt
View information about SNAP-25 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 0    Revision Date: 10.24.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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