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Biotin anti-Ubiquitin Antibody

Pricing & Availability
Clone
P4D1 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P4D1_BIOTIN_Ubiquitin_Antibody_WB_1_110320
Whole cell extracts (15 µg protein) from HeLa cells untreated (-) or treated with 10 µM MG-132 for 10 hours (+) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL (1:4000 dilution) biotin anti-Ubiquitin antibody (clone P4D1) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP streptavidin (Cat. No. 405210) at a 1:20000 dilution. Lane M: Molecular weight marker.
  • P4D1_BIOTIN_Ubiquitin_Antibody_WB_1_110320
    Whole cell extracts (15 µg protein) from HeLa cells untreated (-) or treated with 10 µM MG-132 for 10 hours (+) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL (1:4000 dilution) biotin anti-Ubiquitin antibody (clone P4D1) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP streptavidin (Cat. No. 405210) at a 1:20000 dilution. Lane M: Molecular weight marker.
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646305 25 µg 147 CHF
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646306 100 µg 288 CHF
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Description

Ubiquitin is a highly-conserved small protein expressed in all eukaryotes. It is involved in a post-translation modification of proteins called ubiquitination of proteins by the covalent attachment (via an isopeptide bond) of one or more than one ubiquitin monomers. Ubiqutination controls the protein degradation, stability and intracellular localization of many proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
All Species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Denatured glutaraldehyde cross-linked ubiquitin IgG complex
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody recognizes ubiquitin, polyubiquitin, and ubiquitin-conjugated proteins. The ubiquitin protein is extremley well conserved and thus the antibody has extensive species cross-reactivity from yeast to human. This antibody was specifically developed to detect ubiquitin and ubiquitin-substrate conjugates by immunoblotting. Because the antibody recognizes a denatured epitope, it is known to not work in immunoprecipitation or immunostaining applications.

Application References

(PubMed link indicates BioLegend citation)
  1. Magnaudeix A, et al. 2013. Neurobiol Aging. 34:770. PubMed.
RRID
AB_2888821 (BioLegend Cat. No. 646305)
AB_2888821 (BioLegend Cat. No. 646306)

Antigen Details

Structure
Consists of 76 amino acids and has a molecular mass of about 8.5 kd. Highly conserved among all eukaryotic species.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance, Ubiquitin/Protein Degradation
Molecular Family
Autophagosome Markers
Antigen References

1. Kahana, et al. 1999. Mol. Cell Biol 19:6608
2. Ciechanover A, et al. 1998. EMBO J 17:7151
3. Xu P, et al. 2008. Analytical Chemistry 80:3438

Gene ID
6233 View all products for this Gene ID
UniProt
View information about Ubiquitin on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 01.01.0001

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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