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Biotinylated Recombinant Human CD28H (TMIGD2)-Fc Chimera (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
TMIGD2, Transmembrane And Immunoglobulin Domain Containing 2, IGPR-1, CD28H
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Product Citations
publications
a. Biotin-Recom_Human-CD28H_1_CF
Biotinylated recombinant human CD28H binds to immobilized recombinant human B7-H7 in a dose-dependent manner. ED50 is between 3 – 18 ng/mL.
  • a. Biotin-Recom_Human-CD28H_1_CF
    Biotinylated recombinant human CD28H binds to immobilized recombinant human B7-H7 in a dose-dependent manner. ED50 is between 3 – 18 ng/mL.
  • b. Biotin-Recom_Human-CD28H_2_CF
    Stability Testing for Biotinylated recombinant Human CD28H. Biotinylated recombinant human CD28H was aliquoted in PBS, pH 7.2 at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). After this procedure, the samples were tested for their ability in a binding assay with recombinant human B7-H7. The ED50 for this effect is 3 – 18 ng/mL.
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584404 25 µg 165 CHF
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584406 100 µg 365 CHF
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Description

CD28 homolog (CD28H), also referred to as TMIGD2 (Transmembrane and Immunoglobulin Domain Containing 2) and IGPR-1 (Immunoglobulin-containing and Proline-rich Receptor-1), is a member of the immunoglobulin superfamily. It is predominantly expressed in epithelial and endothelial cells. The CD28H structure consists of an extracellular domain resembling an immunoglobulin V-like (IgV-like) domain, which contains two potential glycosylation sites. It also features a transmembrane segment and a cytoplasmic tail with a tyrosine residue. CD28H was previously recognized both as a co-stimulatory receptor and as an adhesion molecule. HHLA2 (B7-H7) is the known ligand for CD28H. Connection of the CD28H and HHLA2 primarily co-stimulates human T cell growth through an Akt-dependent signaling cascade which mediate tumor resistance against NK cells and promotes cytokine production. CD28H is associated with immune dysregulation and angiogenesis. In autoimmune diseases, abnormal CD28H signaling can cause inappropriate T cell activation, resulting in tissue damage. Additionally, CD28H expression can promote the abnormal self-renewal and differentiation of acute myeloid leukemia cells via downstream signaling pathways like ERK1/2-p90RSK-CREB signaling. Targeting CD28 signaling with anti-CD28 mAb attenuates self-renewal of leukemia stem cells, providing promising therapeutic value. 

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Biotinylated Recombinant Human CD28H, amino acid (Leu23-Gly150) (Accession # Q96BF3.2), with a linker, a C-terminal Human IgG (Pro100-Lys330), and an Avi-tag, was site-specifically biotinylated by enzyme BirA, was expressed in 293E.
Molecular Mass
The 380 amino acid recombinant protein has a predicted molecular mass of approximately 42.46 kD. The DTT-reduced and non-reduced proteins migrate at approximately 50 -70 kD and 75 – 120 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Leu.
Purity
> 95%, as determined by Coomassie-stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2
Endotoxin Level
Less than 0.1 EU per µg of cytokine as determined by the LAL method
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin the vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
When recombinant human B7-H7 is immobilized at 1 µg/mL, biotinylated recombinant human CD28H binds in a dose-dependent manner with ED50 range of 3 – 18 ng/mL. HRP-Avidin (Cat. No. 405103) was used to detect the binding.
Application

Bioassay
Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Dimer
Distribution

NK cell, T cell, epithelial and endothelial cells

Function
Regulates lymphocyte responses, boosts T-cell proliferation, and plays a crucial role in cell migration and the formation of new blood vessels
Interaction
B7-H7/HHLA2
Ligand/Receptor
B7 family
Bioactivity
The bioactivity was measured by immobilized recombinant human B7-H7 binding biotinylated recombinant human CD28H in a dose-dependent manner using the functional ELISA.
Cell Type
Epithelial cells, NK cells, T cells
Biology Area
Angiogenesis, Cancer Biomarkers, Cell Biology, Immuno-Oncology, Immunology
Molecular Family
Adhesion Molecules, Soluble Receptors
Antigen References
  1. Burke Kelly P, et al. 2024. Immunity. 57:223-244.
  2. Zhu Yuwen, et al. 2013. Nat Commun. 4:2043.
  3. Kula,Agnieszka, et al. 2024. Cells. 13:794.
  4. Wang Hao, et al. 2024. Nature communications. 15.11. 
  5. Janakiram Murali, et al. 2017. Immunol Rev. 276:26-39.
  6. Nagai Shigenori and Miyuki Azuma. 2019. Advances in experimental medicine and biology. 1189: 25-51.
  7. Zhong C, et al. 2020. Clinical and experimental immunology. 200:12-21.
  8. Tian Yu, et al. 2018. Oncoimmunology. 8:e1538440.
  9. Ying Honggang, et al. 2022. EBioMedicine. 79:103987.
  10. Amraei Razie, et al. 2020. J Biol Chem. 295:16691-16699.
Gene ID
126259 View all products for this Gene ID
UniProt
View information about CD28H on UniProt.org
Go To Top Version: 1    Revision Date: 10.10.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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