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Biotinylated Recombinant Human VEGF-165 (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
VEGFA, VPF, VEGF-A, VEGF
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Product Citations
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Human_VEGF-165-_CF_BIOTIN-RECOM_111022
Biotinylated recombinant human VEGF-165 binds to immobilized recombinant human VEGFR1 (Cat. No. 555804) at 2 ug/mL in a dose-dependent manner. The ED50 for this effect is 1.5 – 7.5 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
  • Human_VEGF-165-_CF_BIOTIN-RECOM_111022
    Biotinylated recombinant human VEGF-165 binds to immobilized recombinant human VEGFR1 (Cat. No. 555804) at 2 ug/mL in a dose-dependent manner. The ED50 for this effect is 1.5 – 7.5 ng/mL. HRP Avidin (Cat. No. 405103) was used to detect the binding.
  • Human_VEGF-165-_CF_BIOTIN-RECOM_2_111022
    Stability Testing for Biotinylated Recombinant Human VEGF-165. Biotinylated recombinant human VEGF-165 was aliquoted in 5 mM citric acid pH 4, 5 mM Na2HPO4, 0.15M NaCl at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). After this procedure, the samples were tested for their ability to bind to immobilized recombinant human VEGFR1 (Cat. No. 555804) at 2 μg/mL in a dose-dependent manner. The ED50 for this effect is 1.5 – 7.5 ng/mL.
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798204 25 µg 421 CHF
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798206 100 µg 954 CHF
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Description

VEGF (known also as VEGFA) was initially identified in conditioned medium from bovine pituitary follicular cells. VEGFA belongs to the VEGF family, which has the following members: VEGF-A, VEGF-B, VEGF-C (VEGF-2), VEGF-D, and PlGF (placental growth factor). In addition, viral VEGF homologs (collectively called VEGF-E) and snake venom VEGFs, such as Trimeresurus flavoviridis, and svVEGF (called VEGF-F), have been described. VEGFA is alternatively spliced to generate variants with different numbers of amino acids, such as VEGFA121, VEGFA145, VEGFA165, and VEGFA189. VEGFA165 is predominant and responsible for VEGFA biological potency.

While VEGF121 is freely diffusible and does not bind to neuropilins (NRPs) or heparan sulphate (HS), VEGF165 and VEGF189 bind to both, resulting in retention on the cell surface or in the extracellular matrix. NRP1 lacks a typical kinase domain and acts as a co-receptor, and in response to VEGF165, NRP1 couples with VEGF-Rs to signal in endothelial cells. In addition, it has been suggested that bone marrow cells that are recruited to Ewing’s tumors are differentiated into vascular smooth muscle cells, and VEGF165 is responsible for this differentiation.

VEGFA is highly expressed in most of the solid tumors generated in breast, lung, renal, colorectal, and liver tissues. VEGFA has strong vascular permeability activity, and significantly contributes to the formation of ascites tumors. VEGFA can act as a direct proinflammatory mediator during the pathogenesis of rheumatoid arthritis (RA), and protect rheumatoid synoviocytes from apoptosis, which contributes to synovial hyperplasia. VEGFA is expressed in synovial macrophages and synovial fibroblasts in RA patients. Also, VEGFA is associated to age-related macular degeneration (AMD). AMD is due to neovascularization that originates from endothelial cells in the choroid that grow into neurosensory retina as choroidal neovascularization (CNV).

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human VEGF-165, amino acids Ala27-Arg191 (Accession# P15692), with a C-terminal Avi-tag, was expressed in CHO cells. Human VEGF-165-Avi tag was site-specifically biotinylated by enzyme BirA.
Molecular Mass
The 180 amino acid recombinant protein has a predicted molecular mass of approximately 20.97 kD. The DTT-reduced and non-reduced protein migrates at approximately 20-28 kD and 35-40 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Ala.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in 5mM Na2HPO4, 0.15M NaCl, 5mM citric acid pH 4.
Endotoxin Level
Less than 0.1 EU per µg cytokine as determined by the LAL method.
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Biotinylated recombinant human VEGF-165 binds to immobilized recombinant human VEGFR1 (Cat. No. 555804) at 2 ug/mL in a dose-dependent manner. The ED50 for this effect is 1.5 – 7.5 ng/mL. HRP Avidin (Ca. No. 405103) was used to detect the binding.
Application

Bioassay
Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Dimer
Distribution

Widely expressed

Function
Vasculogenesis, blood vessel formation from progenitor cells, angiogenesis, regulates haematopoietic stem cell survival, and induces proliferation and cell migration in endothelial cells during wound healing. Stimulates migration of monocytes and macrophages. VEGF gene expression is upregulated via hypoxia, estrogens, and NF-kB pathways.
Interaction
Embryonic, mesenchymal, neural stem cells, endothelial cells, monocytes and macrophages
Ligand/Receptor
VEGFR1 (Flt-1), VEGFR2 (KDR/Flk-1)
Bioactivity
Biotinylated recombinant humanVEGF-165 binds to recombinant human VEGFR1
Cell Type
Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Angiogenesis, Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References
  1. Conn G, et al. 1990. Proc Natl Acad Sci U S A. 87:1323-7.
  2. Gerber HP, et al. 2002. Nature. 417:954-8.
  3. Shibuya M. 2006. J Biochem Mol Biol. 39:469-78.
  4. Reddy K, et al. 2008. Angiogenesis. 11:257-67.
  5. Shibuya M. 2008. BMB Rep. 41:278-86.
  6. Monaghan-Benson E, et al. 2010. Am J Pathol. 177:2091-102.
  7. Koch S & Claesson-Welsh L. 2012. Cold Spring Harb Perspect Med. 2:a006502.
Gene ID
7422 View all products for this Gene ID
UniProt
View information about VEGF-165 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11.10.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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