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Brilliant Violet 421™ anti-human Aiolos Antibody

Pricing & Availability
Clone
14C4C97 (See other available formats)
Regulatory Status
RUO
Other Names
IKZF3, AIO, ZNFN/A3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
14C4C97_BV421_Aiolos_Antibody_1_050616
Human peripheral blood lymphocytes were stained with CD19 APC, treated with the True-Nuclear™ Transcription Factor Buffer Set, and stained with Aiolos (clone 14C4C97) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • 14C4C97_BV421_Aiolos_Antibody_1_050616
    Human peripheral blood lymphocytes were stained with CD19 APC, treated with the True-Nuclear™ Transcription Factor Buffer Set, and stained with Aiolos (clone 14C4C97) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • 14C4C97_BV421_Aiolos_Antibody_2_050616
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
371009 25 tests 160 CHF
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371010 100 tests 396 CHF
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Description

Aiolos, also known as Ikaros family zinc finger 3 (IKZF3), is a 58kD zinc finger protein involved in hematopoetic-specific transcriptional activation. Aiolos is involved in regulation of gene expression in B lymphocytes through chromatin remodeling and formation of homodimers and heterodimers with other members of the Ikaros family. Deletion of Aiolos has been reported in pre-B-acute lymphocytic leukemia (B-ALL).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial Aiolos recombinant protein (1-130aa) expressed in E. Coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

RRID
AB_2616875 (BioLegend Cat. No. 371009)
AB_2616875 (BioLegend Cat. No. 371010)

Antigen Details

Structure
58kD, zinc finger protein.
Distribution

T and B lymphocytes.

Function
Hematopoetic-specific transcriptional activator and chromatin remodeler.
Interaction
Forms heterodimers with Ikaros family members IKZF4 and IKZF5.
Cell Type
B cells, T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Zhuang Y, et al. 2015. Oncol. Rep. 33:1319.
2. Ma S, et al. 2008. Blood. 111:1396.
3. Billot K, et al. 2010. Leuk. Res. 34:289.
4. Morgan B, et al. 2004. EMBO J. 16:2004.
5. Wang JH, et al. 1998. Immunity. 9:543-53.
6. Hosokawa Y, et al. 1999. Genomics. 61:326.

Gene ID
22806 View all products for this Gene ID
UniProt
View information about Aiolos on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 05.11.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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