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Brilliant Violet 421™ anti-human CD179a (VpreB) Antibody

Pricing & Availability
Clone
HSL96 (See other available formats)
Regulatory Status
RUO
Other Names
VpreB, VPREB1, IGVPB
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HSL96_BV421_CD179a_Antibody_053024
Human pre-B cell line NALM6 was stained with anti-human CD179a (VpreB) (clone HSL96) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
  • HSL96_BV421_CD179a_Antibody_053024
    Human pre-B cell line NALM6 was stained with anti-human CD179a (VpreB) (clone HSL96) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
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347407 25 tests 193 CHF
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347408 100 tests 407 CHF
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Description

CD179a is an 18 kD Ig V-like protein expressed on pro-B and early pre-B cells. It is a member of the Ig gene superfamily, known as VpreB, VPREB1, and IGVPB. CD179a noncovalently associates with CD179b (λ5) to form a surrogate light (SL) chain. The SL chain is complexed with membrane bound IgM heavy (µH) chain and CD79a/CD79b heterodimer to form the pre-B-cell receptor (PreBCR) complex. The function of CD179a/CD179b is still under investigation though it has been reported that the pre-BCR complex plays a critical role in B cell development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Tsyganezawa K, et al. 1998. Blood 92:4317
  2. Kiyokawa N, et al. 2004. Modern Pathology. 17:423
RRID
AB_3662246 (BioLegend Cat. No. 347407)
AB_3662246 (BioLegend Cat. No. 347408)

Antigen Details

Structure
Ig superfamily, Ig V-like, 18 kD, noncovalently associates with CD179b to form surrogate light (SL) chain
Distribution

Pro-B and early Pre-B cells

Cell Type
B cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication
2. Zhang M, et al. 2004. Cell. Mol. Immunol. 1:89

Gene ID
7441 View all products for this Gene ID
UniProt
View information about CD179a on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 05.30.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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