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Brilliant Violet 421™ anti-human CD186 (CXCR6) Antibody

Pricing & Availability
Clone
K041E5 (See other available formats)
Regulatory Status
RUO
Other Names
BONZO, STRL33, TYMSTR
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
K041E5_BV421_CD186_Antibody_FC_1_091213
Human peripheral blood mononuclear cells were stimulated with IL-2 for 9 days, and then stained with CD3 APC and CD186 (clone K041E5) Brilliant Violet 421™ (top) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (bottom).
  • K041E5_BV421_CD186_Antibody_FC_1_091213
    Human peripheral blood mononuclear cells were stimulated with IL-2 for 9 days, and then stained with CD3 APC and CD186 (clone K041E5) Brilliant Violet 421™ (top) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (bottom).
  • K041E5_BV421_CD186_Antibody_FC_2_091213
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
356013 25 tests 215 CHF
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356014 100 tests 435 CHF
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Description

CD186, also known as CXCR6, is a 39 kD, G-protein coupled chemokine receptor, with seven transmembrane-spanning regions. CXCR6 is expressed on activated and memory T cells. Its ligand is the chemokine CXCL16. CXCR6 is involved in the recruitment of cells to inflamed sites and is also a coreceptor for HIV.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
cDNA encoding human CXCR6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Product Citations
  1. Swadling L, et al. 2020. Cell Rep. 30:687. PubMed
  2. Hinks TSC, et al. 2019. Cell Rep. 28:3249. PubMed
  3. Buggert M, et al. 2020. Cell. 183(7):1946-1961.e15. PubMed
RRID
AB_2562514 (BioLegend Cat. No. 356013)
AB_2562514 (BioLegend Cat. No. 356014)

Antigen Details

Structure
GPCR, seven transmembrane-spanning regions, 39 kD
Distribution

Memory T cells, activated T cells

Function
Chemotaxis, coreceptor for HIV
Ligand/Receptor
CXCL16
Cell Type
T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Kim CH, et al. 2001. J. Clin. Invest. 107:595.
2. Nanki T, et al. 2005. Arthritis Rheum. 52:3004.
3. Van der Voort R, et al. 2005. Arthritis Rheum. 52:1381.
4. Aust G, et al. 2005. Eur. J. Endocrinol. 152:635.
5. Agostini C, et al. 2005. Am. J. Respir. Crit. Care Med. 172:1290.
6. Gaida MM, et al. 2008. Clin. Exp. Immunol. 154:216.
7. Huang Y, et al. 2008. J. Immunol. 180:2367.

Gene ID
10663 View all products for this Gene ID
UniProt
View information about CD186 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 1    Revision Date: 09.13.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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