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Brilliant Violet 421™ anti-human CD199 (CCR9) Antibody

Pricing & Availability
Clone
L053E8 (See other available formats)
Regulatory Status
RUO
Other Names
GPR 9-6, GPR28, CDw199, Chemokine Receptor 9
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
L053E8_BV421_CD199_Antibody_070717
Human acute lymphoblastic leukemia cell line, MOLT-4, was stained with CD199 (CCR9) (clone L053E8) Brilliant Violet 421™ (filled histogram) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
  • L053E8_BV421_CD199_Antibody_070717
    Human acute lymphoblastic leukemia cell line, MOLT-4, was stained with CD199 (CCR9) (clone L053E8) Brilliant Violet 421™ (filled histogram) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
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358913 25 tests 182 CHF
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358914 100 tests 402 CHF
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Description

Human CD199, also known as CCR9, is a member of the G protein coupled receptor family and is involved in T cell development in the thymus and in the gut-associated immune response. It is highly expressed on different stages of thymocytes and upregulated on CD4+ CD8+ cells. Expression of CCR9 on γ/δ T cells in the intraepithelial and small intestine has been reported. The interaction of CCR9 with its ligand CCL25 (TECK, thymus-expressed chemokine) may direct the trafficking of developing T cells in the thymus and the generation of gut-specific immunological memory.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Cells transfected with human CCR9
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Product Citations
  1. Liechti T, et al. 2022. Nat Commun. 13:7255. PubMed
RRID
AB_2687387 (BioLegend Cat. No. 358913)
AB_2687387 (BioLegend Cat. No. 358914)

Antigen Details

Structure
A member of G-protein coupled receptors which contains seven transmembrane domains. Human CCR9 has above 80% amino acid sequence homology with its mouse counterpart.
Distribution

CD4+CD8+ thymocytes, γ/δ T cells, subset of memory T cells

Function
May direct the trafficking of developing T cells in the thymus and generation of gut-specific immunological memory; co-receptor for HIV-1
Ligand/Receptor
CCL25
Cell Type
T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Zaballos A, et al. 1999. J. Immunol. 162:5671.
2. Wurbel MA, et al. 2007. J. Immunol. 178:7598.
3. Wurbel MA, et al. 2006. Eur. J. Immunol. 36:73.

Gene ID
10803 View all products for this Gene ID
UniProt
View information about CD199 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 0    Revision Date: 07.07.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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