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Brilliant Violet 421™ anti-human CD4 Antibody

Pricing & Availability
Clone
SK3 (See other available formats)
Regulatory Status
RUO
Other Names
T4, Leu3a
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SK3_BV421_CD4_Antibody_FC_071615
Human peripheral blood lymphocytes were stained with CD4 (clone SK3) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
  • SK3_BV421_CD4_Antibody_FC_071615
    Human peripheral blood lymphocytes were stained with CD4 (clone SK3) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
344631 25 tests 191 CHF
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344632 100 tests 386 CHF
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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Evans RL, et al. 1981. Immunol. 78:544
  2. Arno A et al. 1999. J. Infect. Dis. 180:56
  3. Muech M, et al. 1997. Blood 89:1364
  4. Wang L, et al. 2012. Cytometry A. 81:567. PubMed
Product Citations
  1. Emmons TR, et al. 2021. Cancer Immunol Res. 9:790. PubMed
  2. James KR, et al. 2020. Nat Immunol. 1.113194444. PubMed
  3. Savage AK, et al. 2021. iScience. 24(5):102404. PubMed
  4. Yang Zhou J, et al. 2022. Front Immunol. 13:898473. PubMed
RRID
AB_2566015 (BioLegend Cat. No. 344631)
AB_2566015 (BioLegend Cat. No. 344632)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D et al. 1996. Immunol. Today 17:476.
2. Gaubin M et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD4 Reagents Request Custom Conjugation
Description Clone Applications
PerCP anti-human CD4 SK3 FC
Purified anti-human CD4 SK3 FC,IP
FITC anti-human CD4 SK3 FC
PE anti-human CD4 SK3 FC
PerCP/Cyanine5.5 anti-human CD4 SK3 FC
Biotin anti-human CD4 SK3 FC
PE/Cyanine7 anti-human CD4 SK3 FC
APC anti-human CD4 SK3 FC
APC/Cyanine7 anti-human CD4 SK3 FC
Alexa Fluor® 488 anti-human CD4 SK3 FC
Pacific Blue™ anti-human CD4 SK3 FC
Alexa Fluor® 700 anti-human CD4 SK3 FC
Purified anti-human CD4 (Maxpar® Ready) SK3 FC,CyTOF®
PE anti-human CD4 SK3 FC
Brilliant Violet 421™ anti-human CD4 SK3 FC
Brilliant Violet 510™ anti-human CD4 SK3 FC
Alexa Fluor® 647 anti-human CD4 SK3 FC
FITC anti-human CD4 SK3 FC
APC/Fire™ 750 anti-human CD4 SK3 FC
PE/Dazzle™ 594 anti-human CD4 SK3 FC
Pacific Blue™ anti-human CD4 SK3 FC
Brilliant Violet 785™ anti-human CD4 SK3 FC
Brilliant Violet 750™ anti-human CD4 SK3 FC
Brilliant Violet 605™ anti-human CD4 SK3 FC
Brilliant Violet 711™ anti-human CD4 SK3 FC
PE/Cyanine7 anti-human CD4 SK3 FC
TotalSeq™-A0045 anti-human CD4 SK3 PG
TotalSeq™-C0045 anti-human CD4 SK3 PG
PE/Cyanine5 anti-human CD4 SK3 FC
PerCP/Cyanine5.5 anti-human CD4 SK3 FC
Spark Blue™ 550 anti-human CD4 SK3 FC
Spark NIR™ 685 anti-human CD4 SK3 FC
APC anti-human CD4 SK3 FC
KIRAVIA Blue 520™ anti-human CD4 SK3 FC
APC/Fire™ 810 anti-human CD4 SK3 FC
PE/Fire™ 640 anti-human CD4 SK3 FC
PE/Fire™ 700 anti-human CD4 SK3 FC
PerCP anti-human CD4 SK3 FC
Spark Violet™ 538 anti-human CD4 Antibody SK3 FC
Spark YG™ 581 anti-human CD4 SK3 FC
Alexa Fluor® 660 anti-human CD4 SK3 FC
Spark YG™ 593 anti-human CD4 SK3 FC
PE/Fire™ 810 anti-human CD4 Antibody SK3 FC
APC/Fire™ 750 anti-human CD4 SK3 FC
Spark Blue™ 574 anti-human CD4 SK3 FC
GMP APC anti-human CD4 SK3 FC
TotalSeq™-B0045 anti-human CD4 Antibody SK3 PG
Spark Violet™ 423 anti-human CD4 Antibody SK3 FC
GMP Pacific Blue™ anti-human CD4 SK3 FC
Spark UV™ 387 anti-human CD4 SK3 FC
GMP PE anti-human CD4 SK3 FC
GMP FITC anti-human CD4 SK3 FC
Spark Red™ 718 anti-human CD4 SK3 FC
GMP PerCP anti-human CD4 SK3 FC
GMP PE/Cyanine7 anti-human CD4 SK3 FC
Spark Violet™ 500 anti-human CD4 SK3 FC
GMP PerCP/Cyanine5.5 anti-human CD4 SK3 FC
Brilliant Violet 650™ anti-human CD4 SK3 FC
PerCP/Fire™ 806 anti-human CD4 SK3 FC
Spark Blue™ 515 anti-human CD4 SK3 FC
GMP APC/Fire™ 750 anti-human CD4 SK3 FC
Spark Violet™ 423 anti-human CD4 SK3 FC
PerCP/Fire™ 780 anti-human CD4 SK3 FC
PE/Fire™ 744 anti-human CD4 SK3 FC
Spark PLUS UV395™ anti-human CD4 SK3 FC
PE/Dazzle™ 594 anti-human CD4 SK3 FC
Spark PLUS B550™ anti-human CD4 SK3 FC
Go To Top Version: 1    Revision Date: 07.16.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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